Department of Pharmacy, Eye and ENT Hospital, Fudan University, Shanghai 200031, China.
Department of Laboratory Medicine, Eye and ENT Hospital, Fudan University, Shanghai 200031, China.
J Pharm Biomed Anal. 2024 Dec 15;251:116428. doi: 10.1016/j.jpba.2024.116428. Epub 2024 Aug 22.
Changes in metabolic characteristics are important features of tumor progression and prognosis, including nasopharyngeal carcinoma (NPC). Identifying serum metabolites as potential diagnostic and chemoradiotherapy response biomarkers for NPC is therefore crucial. In this study, ultra-performance liquid chromatography coupled with linear ion trap quadrupole orbitrap high-resolution mass spectrometry (UPLC-LTQ-Orbitrap MS) was used to analyze metabolic variations among controls, NPC patients, and NPC patients undergoing chemoradiotherapy (CRT). Univariate and multivariate analyses revealed seven differential metabolites between the control and NPC groups and eleven metabolites between the CRT and NPC groups. Five common metabolites, gluconic acid, palmitic acid, LysoPC (15:0/0:0), stearic acid, and LysoPC (20:2(11Z,14Z)/0:0), were consistently altered across groups. Notably, the first four metabolites were adjusted closer to normal after chemoradiotherapy, while this change is not reflected at LysoPC (20:2(11Z,14Z)/0:0). These common metabolites were enriched in five pathways. These findings underscore the importance of serum metabolite profiling in NPC diagnosis and treatment response assessment and offer a promising foundation for further clinical research.
代谢特征的改变是肿瘤进展和预后的重要特征，包括鼻咽癌（NPC）。因此，鉴定血清代谢物作为 NPC 的潜在诊断和放化疗反应生物标志物至关重要。在这项研究中，采用超高效液相色谱-线性离子阱四极杆轨道阱高分辨质谱联用技术（UPLC-LTQ-Orbitrap MS）分析了对照组、NPC 患者和接受放化疗（CRT）的 NPC 患者之间的代谢变化。单变量和多变量分析显示，对照组和 NPC 组之间有 7 种差异代谢物，CRT 组和 NPC 组之间有 11 种代谢物。5 种常见的代谢物，葡萄糖酸、棕榈酸、LysoPC（15:0/0:0）、硬脂酸和 LysoPC（20:2(11Z,14Z)/0:0），在各组中均发生改变。值得注意的是，前四种代谢物在放化疗后调整得更接近正常，而 LysoPC（20:2(11Z,14Z)/0:0）则没有这种变化。这些常见的代谢物富集在 5 条通路中。这些发现强调了血清代谢组学在 NPC 诊断和治疗反应评估中的重要性，并为进一步的临床研究提供了有希望的基础。
