Department of Clinical Laboratory, Zhuhai Hospital, Jinan University, Zhuhai, Guangdong, PR China.
Clin Biochem. 2011 Jun;44(8-9):711-8. doi: 10.1016/j.clinbiochem.2011.03.025. Epub 2011 Mar 21.
To search for markers of nasopharyngeal carcinoma (NPC) for diagnosis.
Using gas chromatography and mass spectrometry, we evaluated 51 serum metabolites in 49 NPC, 37 throat cancer patients and 40 healthy controls. High metabolites were selected and confirmed in NPC tissues. Sensitivity and specificity were appraised for 53 NPC diagnoses.
Metabolic profiling revealed that kynurenine, N-acetylglucosaminylamine, N-acetylglucosamine and hydroxyphenylpyruvate increased in NPC patient sera. Their sensitivity and specificity were respectively 79% and 71%, 78% and 69%, 83% and 68%, 84% and 73% for NPC diagnosis. These increases were confirmed in NPC cells. Four metabolites gradually increased from stage I to stage III. After radiotherapy, four metabolites decreased gradually, and tended to a normal level, and were associated with rate of tumor reduction.
The results reveal that kynurenine, N-acetylglucosaminylamine, N-acetylglucosamine and hydroxyphenylpyruvate are potentially markers of NPC for diagnosis and therapy.
寻找鼻咽癌 (NPC) 的诊断标志物。
我们使用气相色谱和质谱联用技术，评估了 49 例 NPC、37 例喉癌患者和 40 例健康对照者的 51 种血清代谢物。在 NPC 组织中对高代谢物进行了选择和验证。对 53 例 NPC 诊断进行了敏感性和特异性评估。
代谢组学分析显示，色氨酸、N-乙酰氨基葡萄糖胺、N-乙酰葡萄糖胺和对羟基苯丙酮酸在 NPC 患者血清中升高。它们对 NPC 的诊断的敏感性和特异性分别为 79%和 71%、78%和 69%、83%和 68%、84%和 73%。这些增加在 NPC 细胞中得到了证实。四种代谢物从 I 期到 III 期逐渐增加。放疗后，四种代谢物逐渐减少，趋于正常水平，并与肿瘤缩小率相关。
结果表明，色氨酸、N-乙酰氨基葡萄糖胺、N-乙酰葡萄糖胺和对羟基苯丙酮酸可能是 NPC 诊断和治疗的潜在标志物。
