CAPZB mRNA 是宫颈高级别鳞状病变的新型生物标志物。
CAPZB mRNA is a novel biomarker for cervical high-grade squamous lesions.
机构信息
Department of Gynaecology, Minhang Hospital, Fudan University, Shanghai, 201199, China.
Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Centre for Systems Biomedicine, Shanghai Jiao Tong University, Shanghai, 200240, China.
出版信息
Sci Rep. 2024 Aug 29;14(1):20047. doi: 10.1038/s41598-024-71112-z.
This study aimed to evaluate the potential of capping protein (actin filament) muscle Z-line subunit β (CAPZB) messenger ribonucleic acid (mRNA) levels as a biomarker for distinguishing low-grade squamous intraepithelial lesions of the cervix (LSIL) from high-grade squamous intraepithelial lesions of the cervix (HSIL). We collected a total of 166 cervical exfoliated cells and divided them into five groups based on histopathological results. Each sample was divided into two portions, one for fluorescence in situ hybridization (FISH) detection and the other for bisulfite sequencing polymerase chain reaction (BSP) detection. We found that FISH detection of CAPZB mRNA mean fluorescence intensity (MFI) and BSP detection of CAPZB deoxyribonucleic acid (DNA) percentage of methylation rate (PMR) performed as biomarkers for distinguishing HSIL from LSIL, with an area under the receiver operating characteristic curve (AUC), sensitivity, specificity and cut-off value of 0.893, 81.25%, 80.39% and 0.616, 0.794, 64.06%, 81.37% and 0.454, respectively. Furthermore, FISH detection of CAPZB mRNA exhibited a greater AUC (0.893) for the detection of HSIL than the CAPZB DNA methylation method (0.794), indicating the CAPZB mRNA levels can be used as a biomarker for assessing cervical lesions.
本研究旨在评估衔接蛋白（肌动蛋白丝）Z 线亚基β（CAPZB）信使核糖核酸（mRNA）水平作为区分宫颈低级别鳞状上皮内病变（LSIL）和高级别鳞状上皮内病变（HSIL）的生物标志物的潜力。我们共收集了 166 例宫颈脱落细胞，根据组织病理学结果将其分为五组。每个样本分为两部分，一部分用于荧光原位杂交（FISH）检测，另一部分用于亚硫酸氢盐测序聚合酶链反应（BSP）检测。我们发现，FISH 检测 CAPZB mRNA 平均荧光强度（MFI）和 BSP 检测 CAPZB 脱氧核糖核酸（DNA）甲基化率（PMR）可作为区分 HSIL 和 LSIL 的生物标志物，其受试者工作特征曲线（ROC）下面积（AUC）、灵敏度、特异性和截断值分别为 0.893、81.25%、80.39%和 0.616、0.794、64.06%、81.37%和 0.454。此外，FISH 检测 CAPZB mRNA 对 HSIL 的检测 AUC（0.893）大于 CAPZB DNA 甲基化方法（0.794），表明 CAPZB mRNA 水平可作为评估宫颈病变的生物标志物。
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