Ulich T R, Anders K, Layfield L, Cheng L, Lewin K J
Arch Pathol Lab Med. 1985 May;109(5):403-7.
Hepatitis B core antigen (HBcAg) was immunohistochemically demonstrated in 19 of 30 needle liver biopsies (63%) of chronic active hepatitis (CAH) from 15 of 24 patients (63%) whose serum contained hepatitis B surface antigen (HBsAg). The percentage of hepatocytes with nuclear and/or cytoplasmic immunoreactivity was quantified in each biopsy specimen, and these percentages were then compared with the amount of HBsAg and the degree of inflammation within the biopsy specimen. The percentage of HBcAg-positive hepatocytes in a biopsy specimen was greatest in those specimens that contained the most HBsAg, although this finding was not statistically significant. The percentage of HBcAg-positive hepatocytes was greatest in those specimens having the least inflammatory activity, and this was statistically significant (P less than .01). Also, the percentage of biopsy specimens containing HBcAg was increased in those groups with lesser inflammatory activity (P less than .01). By staining for both HBcAg and HBsAg, the detection rate of hepatitis B (HB)-related antigens rose to 27 of 30 biopsies (90%) in 22 of 24 patients (92%).
在24例血清中含有乙型肝炎表面抗原（HBsAg）的患者中，有15例（63%）的慢性活动性肝炎（CAH）患者的30份肝脏穿刺活检标本中有19份（63%）经免疫组织化学方法证实存在乙型肝炎核心抗原（HBcAg）。对每个活检标本中具有核和/或细胞质免疫反应性的肝细胞百分比进行了定量，然后将这些百分比与活检标本中的HBsAg量和炎症程度进行比较。活检标本中HBcAg阳性肝细胞的百分比在含有最多HBsAg的标本中最高，尽管这一发现无统计学意义。HBcAg阳性肝细胞的百分比在炎症活动最少的标本中最高，且具有统计学意义（P<0.01）。此外，炎症活动较轻的组中含有HBcAg的活检标本百分比增加（P<0.01）。通过对HBcAg和HBsAg进行染色，24例患者中有22例（92%）的30份活检标本中乙型肝炎（HB）相关抗原的检出率升至27份（90%）。
