化学去磷酸化磷酸化多糖。

Chemical Dephosphorylation of Phosphorylated Polysaccharide.

机构信息

Department of Food Science and Technology, College of Bioresource Sciences, Nihon University, Fujisawa, Kanagawa, Japan.

出版信息

Methods Mol Biol. 2024;2851:143-149. doi: 10.1007/978-1-0716-4096-8_13.

DOI:10.1007/978-1-0716-4096-8_13

PMID:39210179

Abstract

In exocellular polysaccharides produced from lactic acid bacteria with bioactivity, phosphate groups in polysaccharide have been found to act as an active factor. This chapter introduces chemical dephosphorylation using HF by cleaving phosphate diester bonds in extracellular polysaccharides to clarify phosphorus group functions. After this operation, the purified dephospho-polysaccharide can be used for evaluation of bioactivity.

摘要

在具有生物活性的乳酸菌产生的胞外多糖中，已经发现多糖中的磷酸基团起着活性因子的作用。本章介绍了使用 HF 通过切断胞外多糖中的磷酸二酯键进行化学去磷酸化，以阐明磷基团的功能。经过该操作，纯化的去磷酸化多糖可用于生物活性评估。