Vicenzi E, Bianchi M, Salmona M, Donati M B, Poggi A, Ghezzi P
Biochem Biophys Res Commun. 1985 Mar 29;127(3):843-8. doi: 10.1016/s0006-291x(85)80020-6.
Incubation with highly purified human Platelet Derived Growth Factor induced ornithine decarboxylase activity in quiescent NIH 3T3 cells concomitantly with mitogenic stimulation. Pretreatment of cells with a specific ornithine decarboxylase inhibitor, DL-alpha-difluoromethyl-ornithine significantly inhibited the effect of the mitogen on DNA synthesis. These experiments suggest that the mitogenic activity of Platelet Derived Growth Factor, similarly to that of other serum growth factors or tumor promoters, is mediated through rise in polyamine levels.
与高度纯化的人血小板衍生生长因子一起孵育，可在静止的NIH 3T3细胞中诱导鸟氨酸脱羧酶活性，同时伴有促有丝分裂刺激。用特异性鸟氨酸脱羧酶抑制剂DL-α-二氟甲基鸟氨酸对细胞进行预处理，可显著抑制有丝分裂原对DNA合成的作用。这些实验表明，血小板衍生生长因子的促有丝分裂活性，与其他血清生长因子或肿瘤启动子类似，是通过多胺水平的升高介导的。
