Mao Zhichao, Wang Xinyu, Zhao Yongtang, Yang Fei, Qin Qin, Jiang Ruilian
Department of Gastroenterology, Yiling People's Hospital, Yichang, Hubei Province, China.
Department of Cardiovascular, Yiling People's Hospital, Yichang, Hubei Province, China.
Cell Biochem Biophys. 2025 Mar;83(1):429-435. doi: 10.1007/s12013-024-01473-9. Epub 2024 Aug 30.
This article aimed to investigate the mechanism of miR-375 in Hp-induced gastric cancer cells (GCCs) model. Human normal gastric mucosal epithelial cell (GMEC) line GES-1 and human GCCs strain MKN45 were used as research objects. The expression of miR-375 was detected after H.pylori (Hp) infection of GCCs. The cell activity was detected by, 53-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method, and the cell multiplication was determined by cell counting kit-8 (CCK-8) method. Transwell assay was used to detect the effect of cell invasion and migration ability. The expression levels of JAK1 and STAT3 proteins were determined by Western blot (WB). MiR-375 was increased in GCCs after Hp infection, and JAK1, STAT3, p-JAK1, and p-STAT3 in GCCs after Hp infection were visibly increased. In addition, the overexpressed miR-375 promoted the multiplication activity, migration, and invasion ability of GCCs. MiR-375 promotes Hp-induced migration and invasion of GCCs by targeting JAK1/STAT3. This article reveals the important role of miR-375 in Hp-induced GC, which provides new clues for further study of its mechanism and therapeutic targets.
本文旨在研究miR-375在幽门螺杆菌（Hp）诱导的胃癌细胞（GCCs）模型中的作用机制。以人正常胃黏膜上皮细胞（GMEC）系GES-1和人GCCs株MKN45为研究对象。检测GCCs感染Hp后miR-375的表达。采用噻唑蓝（MTT）法检测细胞活性，采用细胞计数试剂盒-8（CCK-8）法测定细胞增殖情况。采用Transwell实验检测细胞侵袭和迁移能力。通过蛋白质免疫印迹法（WB）检测JAK1和STAT3蛋白的表达水平。Hp感染后GCCs中miR-375表达升高，且Hp感染后GCCs中JAK1、STAT3、p-JAK1和p-STAT3明显升高。此外，过表达的miR-375促进了GCCs的增殖活性、迁移和侵袭能力。miR-375通过靶向JAK1/STAT3促进Hp诱导的GCCs迁移和侵袭。本文揭示了miR-375在Hp诱导的胃癌中的重要作用，为进一步研究其机制和治疗靶点提供了新线索。
