Biomedical Nanoengineering Laboratory, College of Medicine and Public Health, Flinders University, Bedford Park, South Australia 5042, Australia.
Academic Unit of STEM, University of South Australia, Mawson Lakes, Adelaide, South Australia 5095, Australia.
ACS Biomater Sci Eng. 2024 Oct 14;10(10):6230-6240. doi: 10.1021/acsbiomaterials.4c01103. Epub 2024 Aug 30.
The adsorption of serum proteins on biomaterial surfaces is a critical determinant for the outcome of medical procedures and therapies, which involve inserting materials and devices into the body. In this study, we aimed to understand how surface topography at the nanoscale influences the composition of the protein corona that forms on the (bio)material surface when placed in contact with serum proteins. To achieve that, we developed nanoengineered model surfaces with finely tuned topography of 16, 40, and 70 nm, overcoated with methyl oxazoline to ensure uniform outermost chemistry across all surfaces. Our findings revealed that within the studied height range, surface nanotopography had no major influence on the overall quantity of adsorbed proteins. However, significant alterations were observed in the composition of the adsorbed protein corona. For instance, clusterin adsorption decreased on all the nanotopography-modified surfaces. Conversely, there was a notable increase in the adsorption of ApoB and IgG gamma on the 70 nm nanotopography. In comparison, the adsorption of albumin was greater on surfaces that had a topography scale of 40 nm. Analysis of the gene enrichment data revealed a reduction in protein adsorption across all immune response-related biological pathways on nanotopography-modified surfaces. This reduction became more pronounced for larger surface nanoprotrusions. Macrophages were used as representative immune cells to assess the influence of the protein corona composition on inflammatory outcomes. Gene expression analysis demonstrated reduced inflammatory responses on the nanotopographically modified surface, a trend further corroborated by cytokine analysis. These findings underscore the potential of precisely engineered nanotopography-coated surfaces for augmenting biomaterial functionality.
生物材料表面的血清蛋白吸附是影响医疗程序和治疗结果的关键决定因素，这些程序和治疗涉及将材料和设备插入体内。在这项研究中，我们旨在了解纳米尺度表面形貌如何影响在与血清蛋白接触时形成的（生物）材料表面的蛋白质冠的组成。为了实现这一目标，我们开发了具有精细调谐的 16、40 和 70nm 形貌的纳米工程模型表面，并覆盖了甲基恶唑啉以确保所有表面的最外层化学性质均匀。我们的研究结果表明，在所研究的高度范围内，表面纳米形貌对吸附蛋白的总量没有重大影响。然而，在吸附的蛋白质冠的组成上观察到了显著的变化。例如，在所有纳米形貌修饰的表面上，簇蛋白的吸附减少。相反，在 70nm 纳米形貌上，ApoB 和 IgG gamma 的吸附显著增加。相比之下，在具有 40nm 形貌的表面上，白蛋白的吸附量更大。基因富集数据分析显示，在纳米形貌修饰的表面上，所有与免疫反应相关的生物学途径的蛋白质吸附减少。对于较大的表面纳米突起，这种减少变得更加明显。巨噬细胞被用作代表性免疫细胞来评估蛋白质冠组成对炎症结果的影响。基因表达分析表明，在纳米形貌修饰的表面上炎症反应减少，这一趋势通过细胞因子分析进一步得到证实。这些发现强调了精确工程化的纳米形貌涂层表面在增强生物材料功能方面的潜力。
