Pereyra Nicolas, Yu Kai, Rogalski Jason, Da Silva Taylor Olivia, Golesorkhi Parisa, Goldstein Madeleine, Devine Dana V, Kizhakkedathu Jayachandran N
Centre for Blood Research and Life Sciences Institute, University of British Columbia, 2350 Health Sciences Mall, Vancouver, BC, V6T 1Z3, Canada.
Department of Biochemistry & Molecular Biology, University of British Columbia, 2350 Health Sciences Mall, Vancouver, BC, V6T 1Z3, Canada.
Adv Healthc Mater. 2025 Sep;14(23):e2501217. doi: 10.1002/adhm.202501217. Epub 2025 Jun 23.
Platelet transfusion is an indispensable therapy used in contexts ranging from hemorrhagic bleeding to chemotherapy. However, platelet shelf life and quality deteriorate during storage in a process termed the platelet storage lesion (PSL), resulting in chronic shortages globally. The PSL is thought to be partially attributed to the bio-incompatible polyvinyl chloride (PVC) storage bags that promote protein fouling and platelet adhesion. Developing platelet-friendly materials is therefore essential for improving platelet storage quality. This study aimed to delineate the contributions of protein adsorption and platelet adhesion to the progression of the PSL. Hydrophilic coatings are screened to identify those which resist protein and platelet adhesion most effectively. These coatings are translated into mini-platelet bags and tested in long-term standard blood banking conditions. Significant reductions in platelet adhesion after 7-day storage do not affect platelet quality. Proteomic characterization of the bag surfaces revealed dynamic changes in the protein coronas on coated and uncoated bag over time, but are uncorrelated with platelet quality. This research demonstrates a method to identify platelet-compatible coatings suitable for long-term storage, as well as a novel approach to characterize the surface of blood storage bags.
血小板输注是一种不可或缺的治疗方法，应用于从出血性出血到化疗等各种情况。然而，在储存过程中，血小板的保质期和质量会在一个称为血小板储存损伤（PSL）的过程中恶化，导致全球范围内长期短缺。PSL被认为部分归因于促进蛋白质污染和血小板粘附的生物不相容聚氯乙烯（PVC）储存袋。因此，开发对血小板友好的材料对于提高血小板储存质量至关重要。本研究旨在阐明蛋白质吸附和血小板粘附对PSL进展的贡献。筛选亲水性涂层以确定那些最有效地抵抗蛋白质和血小板粘附的涂层。将这些涂层转化为微型血小板袋，并在长期标准血库条件下进行测试。储存7天后血小板粘附的显著减少不会影响血小板质量。袋表面的蛋白质组学表征揭示了有涂层和无涂层袋上蛋白质冠随时间的动态变化，但与血小板质量无关。这项研究展示了一种识别适合长期储存的血小板相容性涂层的方法，以及一种表征血液储存袋表面的新方法。
