Nicolaus Copernicus University in Toruń, Collegium Medicum in Bydgoszcz, Department of Medical Biology and Biochemistry, Division of Ecology and Environmental Protection, M. Skłodowska-Curie St. 9, Bydgoszcz PL 85-094, Poland.
Nicolaus Copernicus University in Toruń, Collegium Medicum in Bydgoszcz, Department of Medical Biology and Biochemistry, Division of Ecology and Environmental Protection, M. Skłodowska-Curie St. 9, Bydgoszcz PL 85-094, Poland; University of Zielona Góra, Faculty of Biological Sciences, Institute of Biological Sciences, Department of Biotechnology, Prof. Z. Szafran St. 1, Zielona Góra PL 65-516, Poland.
Ecotoxicol Environ Saf. 2024 Oct 1;284:116919. doi: 10.1016/j.ecoenv.2024.116919. Epub 2024 Aug 29.
Male infertility is a world multifactorial problem modulated by environmental and genetic factors. Male aspects account for 20-50 % of infertility cases. Our results are unique because they treat the importance of components participating in the determination of male infertility (environmental and immunogenetic determinants, seminological analysis, lipoperoxidation, genetic determinants, role of aluminum, arsenic, cadmium and boron). We analyzed agents affecting male reproductive potential (aluminum, boron, cadmium, arsenic, lipid peroxidation, gene polymorphisms (MTHFRv.C677T (rs1801133) (chromosome-1) and IL-4v.C589T (rs2243250) (chromosome-5) in men with semen disorders (n=76) and with normozoospermia (n=87) from Central Poland. Polymorphisms of MTHFRv.C677T and IL-4v.C589T genes indirectly shape toxic metals concentration and lipoperoxidation but do not exert direct influence on male fertility disorders (monomorphism and lack of differences in genotypes frequency). Men with genotype TT or CC (IL-4v.C589T) show some differentiation in elements concentration and intensity of lipoperoxidation. Analysis of TT or CC (IL-4v.C589T) genotype brought correlations with B, Al, Cd, and lipoperoxidation (P<0.05) and suggesting that mentioned factors jointly shape male reproductive capability. Toxic metals may play an important role in shaping of men genetic polymorphisms, since Cd was identified as a factor increasing risk of qualification to infertile group, predisposing to fertility disorders. B, Al and Cd may be considered as important modulators of reproductive condition. However, lipoperoxidation as an isolated predictive parameter does not produce convincing results in male reproductive potential (higher MDA concentration in healthy men). Our results may be helpful in the diagnosis of male infertility, in the reduction of idiopathic cases of unknown origin and in implementation of targeted and more effective treatment (pharmacological, hormonal). Identification of environmental stressors and their correlations with fertility disorders can help to eliminate or reduce the impact of factors unfavorable to fertility. Our results highlight the importance of environmental and immunogenetic factors in shaping of defensive potential against destruction of spermatozoa and infer a role of oxidative stress in the induction of gene polymorphisms, affecting male fertility.
男性不育是一个由环境和遗传因素共同作用的世界性多因素问题。男性因素占不育病例的 20-50%。我们的研究结果是独特的，因为它们涉及到参与男性不育症（环境和免疫遗传决定因素、精液分析、脂质过氧化、遗传决定因素、铝、砷、镉和硼的作用）的确定的各个组成部分的重要性。我们分析了影响男性生殖潜力的因素（铝、硼、镉、砷、脂质过氧化、基因多态性（MTHFRv.C677T(rs1801133)（染色体-1）和 IL-4v.C589T(rs2243250)（染色体-5））在波兰中部有精液障碍的男性（n=76）和正常精子症的男性（n=87）中的作用。MTHFRv.C677T 和 IL-4v.C589T 基因的多态性间接影响有毒金属浓度和脂质过氧化，但对男性生育障碍没有直接影响（单态性和基因型频率无差异）。TT 或 CC（IL-4v.C589T）基因型的男性在元素浓度和脂质过氧化强度上有一些差异。TT 或 CC（IL-4v.C589T）基因型的分析与 B、Al、Cd 和脂质过氧化（P<0.05）有关，并表明这些因素共同影响男性生殖能力。有毒金属可能在塑造男性遗传多态性方面发挥重要作用，因为 Cd 被确定为增加不合格为不育组的风险因素，易导致生育障碍。B、Al 和 Cd 可被视为生殖状况的重要调节剂。然而，作为一个孤立的预测参数，脂质过氧化在男性生殖潜能方面没有产生令人信服的结果（健康男性 MDA 浓度较高）。我们的研究结果有助于男性不育的诊断，减少不明原因的特发性病例，并实施靶向和更有效的治疗（药理学、激素）。确定环境压力源及其与生育障碍的相关性有助于消除或减少不利于生育的因素的影响。我们的研究结果强调了环境和免疫遗传因素在塑造精子防御潜力方面的重要性，并推断氧化应激在诱导影响男性生育能力的基因多态性方面的作用。
