Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Division of Physiology, Department of Basic Sciences, Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Poult Sci. 2024 Nov;103(11):104166. doi: 10.1016/j.psj.2024.104166. Epub 2024 Aug 6.
This novel study investigated the effects of intracerebroventricular (ICV) injection α- klotho and its interaction with neuropeptide Y (NPY) receptors on food intake in broiler chicken. This study included 4 experiments with 4 groups in each with 11 replicates per group. Birds were feed deprived 3 h prior injection, following injection returned to their cage and food provided. In experiment 1, group 1 received ICV injection of the saline and groups 2 to 4 received ICV injection of the α-klotho (1, 2, and 4 µg), respectively. In experiment 2, chicken received ICV injection of the saline, B5063 (NPY receptor antagonist, 1.25 µg), α-klotho (4 µg) and co-injection of the B5063 + α-klotho. In experiments 3 and 4, SF22 (NPY receptor antagonist, 1.25 µg), and SML0891 (NPY receptor antagonist, 1.25 µg) were injected instead of the B5063. Then consumed food was measured at 30, 60, and 120 min post the injection. Based on results, ICV injection of the α-klotho (2 and 4 µg) significantly decreased food intake (P < 0.05). Co-injection of the B5063 + α-klotho significantly amplified hypophagic effect of the α-klotho (P < 0.05). α-klotho-induced hypophagia was not influenced by SF22 or SML0891. These results suggest that α-klotho-induced hypophagia is mediated via NPY receptors in broiler chicken.
这项新研究调查了脑室内（ICV）注射α-klotho 及其与神经肽 Y（NPY）受体相互作用对肉鸡采食量的影响。这项研究包括 4 个实验，每个实验有 4 个组，每组有 11 个重复。在注射前，鸟类禁食 3 小时，注射后返回笼子并提供食物。在实验 1 中，第 1 组接受脑室内注射生理盐水，第 2 组至第 4 组分别接受脑室内注射α-klotho（1、2 和 4 µg）。在实验 2 中，鸡接受脑室内注射生理盐水、B5063（NPY 受体拮抗剂，1.25 µg）、α-klotho（4 µg）和 B5063+α-klotho 共注射。在实验 3 和 4 中，SF22（NPY 受体拮抗剂，1.25 µg）和 SML0891（NPY 受体拮抗剂，1.25 µg）代替 B5063 注射。然后在注射后 30、60 和 120 分钟测量消耗的食物量。结果表明，脑室内注射α-klotho（2 和 4 µg）显著降低了采食量（P < 0.05）。B5063+α-klotho 的共注射显著放大了α-klotho 的致厌食作用（P < 0.05）。SF22 或 SML0891 不影响α-klotho 诱导的厌食。这些结果表明，α-klotho 诱导的厌食是通过肉鸡的 NPY 受体介导的。
