Gülhane Education and Research Hospital, Department of Hematology and Bone Barrow Transplantation Unit, Ankara, Turkiye.
Gülhane Education and Research Hospital, Department of Hematology and Bone Barrow Transplantation Unit, Ankara, Turkiye.
Transpl Immunol. 2024 Oct;86:102111. doi: 10.1016/j.trim.2024.102111. Epub 2024 Aug 29.
Effective mobilization of Stem Cells(SCs) to peripheral blood (PB) is crucial for obtaining sufficient CD34 cell numbers via apheresis. The ratio of pre-apheresis PB CD34 cells is the best parameter for predicting the product CD34 cell count. However, quantitating CD34 PB cells requires flow cytometry, which usually takes two or more hours to obtain the results. We hypothesized that the product CD34 cell count could be predicted using the counts of white blood cells (WBCs), mononuclear cells (MNCs), and pre-apheresis CD34 cells. A formula that achieves this would substantially affect the efficiency and effectiveness of apheresis. We, therefore, aimed to estimate the number of CD34 cells in the product using a formula that incorporates pre-apheresis PB WBC, MNC, and CD34 cell counts and product WBC and MNC counts.
We examined the results of 373 leukapheresis procedures for SC mobilization. Effective separation of CD34 PBSCs (count/μL) via apheresis was estimated using the following formula: [Product WBC (count/μL) × MNC (count/μL) × pre-apheresis CD34 cell (percentage/μL)] ÷ [PB WBC count/μL × PB MNC (count/μL)].
A strong correlation was observed between the CD34 cell count calculated using our formula and the post-apheresis CD34 cell count measured via flow cytometry (R = 0.939, based on linear regression analysis). In the subgroup analysis, this correlation was observed for all the disease subgroups and healthy donors.
We developed a formula that predicts the product CD34 cell count and is useful for determining whether a second apheresis procedure will be required.
有效地将干细胞（SCs）动员到外周血（PB）中对于通过单采术获得足够数量的 CD34 细胞至关重要。预单采 PB CD34 细胞的比例是预测产品 CD34 细胞计数的最佳参数。然而，定量 PB 中的 CD34 细胞需要流式细胞术，通常需要两个多小时才能获得结果。我们假设可以使用白细胞（WBC）、单核细胞（MNC）和预单采 CD34 细胞的计数来预测产品 CD34 细胞计数。实现这一目标的公式将极大地影响单采术的效率和效果。因此，我们旨在使用纳入预单采 PB WBC、MNC 和 CD34 细胞计数以及产品 WBC 和 MNC 计数的公式来估计产品中 CD34 细胞的数量。
我们检查了 373 次用于 SC 动员的白细胞单采术的结果。通过以下公式估计通过单采术有效分离 CD34 PBSCs（每微升计数/μL）：[产品 WBC（计数/μL）× MNC（计数/μL）×预单采 CD34 细胞（百分比/μL）]÷[PB WBC 计数/μL× PB MNC（计数/μL）]。
我们公式计算的 CD34 细胞计数与通过流式细胞术测量的单采后 CD34 细胞计数之间存在很强的相关性（基于线性回归分析，R=0.939）。在亚组分析中，这种相关性在所有疾病亚组和健康供体中均观察到。
我们开发了一种预测产品 CD34 细胞计数的公式，有助于确定是否需要进行第二次单采术。
