Sato H, Kidaka T, Hori M
Int J Artif Organs. 1985 Mar;8(2):109-14.
Propylene oxide (PO), in the form of its aqueous solution, was evaluated as a chemical sterilant for therapeutic immunoadsorbent to be used in on-line plasmapheresis. Generally, PO is characterized as less toxic and less antimicrobial than ethylene oxide. PO was significant sporicidal to B. subtilis at 25 degrees C and 10(6) spores/ml were killed with 1% (V/V) PO solution after storage for 3 weeks when 75% to 80% of PO decomposed; the death-rate conformed to the kinetics of a first-order reaction although PO decomposed rapidly. Thus an apparent D values were estimated to be 13 to 16 days with 0.5% PO solution and 5 to 7 days with 1% PO. An immunoadsorbent consisting of Sepharose 4B immobilizing anti-human IgE antibody was used to study the effect of PO on adsorption characteristics. PO affected the IgE removal of the immunoadsorbents depending on its concentration ranging from 0% to 4%. The IgE-adsorptions were lowered by only 17% and 30% of the intact and glutaraldehyde-treated immunoadsorbents respectively after storage for 3 months in the 2% PO solution at 25 degrees C, and these numbers are considered to be acceptable for a clinical use. A 2% PO solution was chosen based on the results of the shelf life test of immunoadsorbents and the sporicidal test of PO.
环氧丙烷（PO）以其水溶液的形式，被评估作为一种化学消毒剂用于在线血浆置换中使用的治疗性免疫吸附剂。一般来说，PO的特点是毒性比环氧乙烷小，抗菌性也比环氧乙烷弱。PO在25℃时对枯草芽孢杆菌具有显著的杀孢子作用，当75%至80%的PO分解后，1%（V/V）的PO溶液在储存3周后可杀死10⁶个孢子/ml；尽管PO分解迅速，但死亡率符合一级反应动力学。因此，0.5%的PO溶液的表观D值估计为13至16天，1%的PO溶液的表观D值估计为5至7天。使用固定有抗人IgE抗体的琼脂糖4B组成的免疫吸附剂来研究PO对吸附特性的影响。PO对免疫吸附剂的IgE去除效果取决于其浓度，范围从0%到4%。在25℃下于2%的PO溶液中储存3个月后，完整的和经戊二醛处理的免疫吸附剂的IgE吸附量分别仅降低了17%和30%，这些数值被认为对于临床应用是可以接受的。基于免疫吸附剂的保质期测试和PO的杀孢子测试结果，选择了2%的PO溶液。
