Department of Chemistry, Xinzhou Normal University, Xinzhou, Shanxi 034000, PR China.
School of Laboratory Medicine, Youjiang Medical University for Nationalities, Baise 533000 Guangxi, PR China.
Bioorg Chem. 2024 Nov;152:107768. doi: 10.1016/j.bioorg.2024.107768. Epub 2024 Aug 28.
Alzheimer's disease is associated both with imbalances in Al production and changes in viscosity in cells. Their simultaneous measurement could therefore provide valuable insights into Alzheimer's disease pathology. Their simultaneous measurement would therefore be of great value in investigating the pathological mechanism of Alzheimer's disease. We designed a fluorescent probe YM2T with AIE effect that is capable of selectively responding to Al by fluorescence colormetrics and to viscosity by fluorescence "turn on" modes. Additionally, Al and viscosity were simultaneously detected in PC12 cells using the low cytotoxic probe YM2T via blue and green fluorescence channels. More importantly, the YM2T probe was used to image mice with AD. Hence, the YM2T probe shows potential as a useful molecular instrument for studying the pathological impact of Al and viscosity.
阿尔茨海默病与 Al 产量的失衡和细胞粘度的变化都有关联。因此，同时测量这两个因素可以为阿尔茨海默病的病理机制提供有价值的见解。因此，同时测量这两个因素对于研究阿尔茨海默病的病理机制具有重要意义。我们设计了一种具有 AIE 效应的荧光探针 YM2T，它能够通过荧光比色法选择性地响应 Al，并通过荧光“开启”模式响应粘度。此外，我们还通过蓝色和绿色荧光通道，使用低细胞毒性探针 YM2T 在 PC12 细胞中同时检测 Al 和粘度。更重要的是，我们使用 YM2T 探针对 AD 小鼠进行了成像。因此，YM2T 探针有望成为一种有用的分子工具，用于研究 Al 和粘度的病理影响。
