Singamshetty Santoshkumar, Selvapandian Upasna, Selvamani Selva Babu, Talya Chandrashekara Suman, Pathak Jyoti, Agarwal Aditi, Thiruvengadam Venkatesan, Ramasamy Gandhi Gracy, Sushil Satya Nand, Kamanur Muralimohan, Nara Nagesha, Mohan Muthugounder
ICAR-National Bureau of Agricultural Insect Resources, Bengaluru 560 024, India; University of Agricultural Sciences, GKVK, Bengaluru 560 065, India.
ICAR-National Bureau of Agricultural Insect Resources, Bengaluru 560 024, India.
Comp Biochem Physiol Part D Genomics Proteomics. 2024 Dec;52:101316. doi: 10.1016/j.cbd.2024.101316. Epub 2024 Aug 22.
Insecticide resistance is a global concern and requires immediate attention to manage dreadful insect pests. One of the resistance mechanisms adopted by insects is through ATP-binding cassette (ABC) transporter proteins. These proteins rapidly transport and eliminate the insecticidal molecules across the lipid membranes (Phase III detoxification mechanism). In the present study, we investigated the potential role of ABC transporter genes in imparting insecticide resistance in field-collected insecticide resistant larvae of eggplant shoot and fruit borer (Leucinodes orbonalis; Crambidae: Lepidoptera). Dose-mortality bioassays against five insecticidal molecules revealed moderate to high levels of insecticide resistance (32.2. to 134.1-fold). Thirty-one genes encoding ABC transporter proteins were mined from the transcriptome resources of L. orbonalis. They were classified under eight sub-families (ABCA to ABCH). Phylogenetic analysis indicated ABCG is the most divergent, composed of nine genes as compared to many other insects. Transcriptome analysis of the insecticide resistant and susceptible strains of L. orbonalis revealed differential expression of 13 ABC transporter genes. The altered expression of these genes was further validated using qRT-PCR. The knockdown studies indicated the involvement of ABCD1 and ABCG2 genes in chlorantraniliprole resistance in the insecticide-resistant strain of L. orbonalis. This study unveils the additional mechanisms employed by L. orbonalis in resisting insecticide toxicity through accelerated excretion mode. These ABCD and ABCG family genes could be candidate targets in developing genome-assisted pest management strategies in the future.
杀虫剂抗性是一个全球性问题，需要立即加以关注以应对可怕的害虫。昆虫采用的一种抗性机制是通过ATP结合盒（ABC）转运蛋白。这些蛋白能迅速将杀虫分子转运并排出脂质膜（III期解毒机制）。在本研究中，我们调查了ABC转运蛋白基因在赋予田间采集的茄子蛀茎蛀果蛾（Leucinodes orbonalis；螟蛾科：鳞翅目）抗药性幼虫杀虫剂抗性方面的潜在作用。针对五种杀虫分子的剂量 - 死亡率生物测定显示出中度至高度的杀虫剂抗性（32.2至134.1倍）。从茄子蛀茎蛀果蛾的转录组资源中挖掘出31个编码ABC转运蛋白的基因。它们被归类为八个亚家族（ABCA至ABCH）。系统发育分析表明ABCG是最具差异的，与许多其他昆虫相比，它由九个基因组成。茄子蛀茎蛀果蛾抗药性和敏感品系的转录组分析揭示了13个ABC转运蛋白基因的差异表达。这些基因的表达变化通过qRT-PCR进一步验证。敲低研究表明ABCD1和ABCG2基因参与了茄子蛀茎蛀果蛾抗药性品系对氯虫苯甲酰胺的抗性。本研究揭示了茄子蛀茎蛀果蛾通过加速排泄模式抵抗杀虫剂毒性所采用的额外机制。这些ABCD和ABCG家族基因可能是未来制定基因组辅助害虫管理策略的候选靶点。
