School of Life Sciences, Chongqing University, Chongqing 401331, China.
Chongqing Key Laboratory of Vector Insects, Institute of Entomology and Molecular Biology, Chongqing Normal University, Chongqing 401331, China.
Int J Mol Sci. 2019 Mar 20;20(6):1409. doi: 10.3390/ijms20061409.
: The ATP-binding cassette (ABC) transporters family is one of the largest families of membrane proteins existing in all living organisms. Pyrethroid resistance has become the largest unique obstacle for mosquito control worldwide. ABC transporters are thought to be associated with pyrethroid resistance in some agricultural pests, but little information is known for mosquitoes. Herein, we investigated the diversity, location, characteristics, phylogenetics, and evolution of ABC transporter family of genes in the genome, and identified the ABC transporter genes associated with pyrethroid resistance through expression profiles using RNA-seq and qPCR. Results: 61 ABC transporter genes are identified and divided into eight subfamilies (ABCA-H), located on 22 different scaffolds. Phylogenetic and evolution analyses with ABC transporters of , , and suggest that the ABCD, ABCG, and ABCH subfamilies are monophyly, and that the ABCC and ABCG subfamilies have experienced a gene duplication event. Both RNA-seq and qPCR analyses show that the gene is uniquely significantly upregulated gene in all three field pyrethroid-resistant populations (Anhui, Chongqing, and Yunnan provinces) in comparison with a laboratory-susceptible strain from Jiangsu province. The is significantly upregulated at 12-h and 24-h after deltamethrin exposure in three-day-old female adults. Conclusion: This study provides the information frame for ABC transporter subfamily of genes, and lays an important basis for the better understanding and further research of ABC transporter function in insecticide toxification. The gene is associated with pyrethroid detoxification, and it functions at later period in the detoxification process for xenobiotics transportation.
: 三磷酸腺苷结合盒（ABC）转运蛋白家族是所有生物中存在的最大的膜蛋白家族之一。拟除虫菊酯抗性已成为全球蚊虫控制的最大独特障碍。ABC 转运蛋白被认为与一些农业害虫的拟除虫菊酯抗性有关，但对蚊子的了解甚少。在此，我们通过 RNA-seq 和 qPCR 研究了基因组中 ABC 转运蛋白家族基因的多样性、位置、特征、系统发生和进化，并通过表达谱鉴定了与拟除虫菊酯抗性相关的 ABC 转运蛋白基因。结果：鉴定出 61 个 ABC 转运蛋白基因，分为 8 个亚家族（ABCA-H），位于 22 个不同的支架上。与 、 、 和 的 ABC 转运蛋白的系统发生和进化分析表明，ABCD、ABCG 和 ABCH 亚家族是单系的，而 ABCC 和 ABCG 亚家族经历了基因复制事件。RNA-seq 和 qPCR 分析均表明，与来自江苏省的实验室敏感株相比，在所有三个田间拟除虫菊酯抗性种群（安徽、重庆和云南）中， 基因是唯一显著上调的基因。在 3 日龄雌性成虫中，双甲脒暴露 12 小时和 24 小时后， 基因显著上调。结论：本研究为 ABC 转运蛋白亚家族基因提供了信息框架，为进一步研究昆虫解毒中 ABC 转运蛋白的功能奠定了重要基础。 基因与拟除虫菊酯解毒有关，在异生物质运输的解毒过程中，它在后期起作用。
