Key Lab of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, China; University of Chinese Academy of Sciences, Beijing, 100049, China.
Key Lab of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, China; Ganjiang Chinese Medicine Innovation Center, Nanchang, 330000, China.
J Chromatogr A. 2024 Oct 11;1734:465315. doi: 10.1016/j.chroma.2024.465315. Epub 2024 Aug 27.
Hydrophilic interaction liquid chromatography (HILIC) based on polar stationary phases has vital research significance in the separation of polar compounds. Numerous HILIC stationary phases with different structures have been developed, which do not have universal properties and broad selectivity, making it a challenge to select the suitable column based on the properties of the samples. Consequently, it is particularly important to develop a bonded phase capable of separating a wide variety of samples, while having enhanced retention, improved selectivity, symmetric peak shape and good stability. Herein, a novel nitrogen-containing heterocyclic bonded phase with multiple functionalities, such as thioether, amino and hydroxyl groups (named AMTA) was employed as HILIC stationary phase. Detailed chromatographic evaluations were carried out, and the results showed that it was superior to other hydrophilic chromatographic columns in terms of selectivity, peak shapes and practical sample separation. Lastly, it has been verified that AMTA exhibited high orthogonality with the XBridge C18 column of reversed-phase liquid chromatography (RPLC) mode. In summary, we anticipate our assay to be instructive to other researchers in developing the HILIC stationary phase.
亲水作用色谱（HILIC）基于极性固定相，在分离极性化合物方面具有重要的研究意义。已经开发出了许多具有不同结构的 HILIC 固定相，但它们没有普遍的性质和广泛的选择性，因此根据样品的性质选择合适的柱子具有一定的挑战性。因此，开发一种能够分离多种样品的键合相，同时具有增强的保留、改善的选择性、对称的峰形和良好的稳定性，这一点尤为重要。本文中，我们使用了一种新型的含氮杂环键合相，其具有硫醚、氨基和羟基等多种功能基团（命名为 AMTA），将其作为 HILIC 固定相。详细的色谱评价表明，与其他亲水色谱柱相比，该固定相在选择性、峰形和实际样品分离方面具有优势。最后，还验证了 AMTA 与反相液相色谱（RPLC）模式的 XBridge C18 柱具有较高的正交性。总之，我们期望我们的研究结果能够为其他研究人员开发 HILIC 固定相提供指导。
