Lin M, Prescott D M
J Protozool. 1985 Feb;32(1):144-9. doi: 10.1111/j.1550-7408.1985.tb03028.x.
The synthesis of DNA in two hypotrichous ciliates, Styx sp. and an amicronucleated strain of Oxytricha sp., was studied by high voltage (1000 kV) electron microscopy. High voltage EM permits use of thick sections (0.25-0.40 micron), including serial sections; thick sections produce strong autoradiographic images with relatively short exposure times. The autoradiographs show that DNA synthesis occurs in a narrow part of the rear zone of a replication band in the macronucleus. Macronuclear DNA synthesis occupies a substantial part of the interdivision interval, and micronuclear DNA synthesis in Styx sp. takes place in early prophase at a time when macronuclear DNA synthesis is in its terminal phase.
利用高压（1000千伏）电子显微镜研究了两种具毛腹毛目纤毛虫——Styx sp.和一种无小核的尖毛虫属菌株的DNA合成。高压电子显微镜允许使用厚切片（0.25 - 0.40微米），包括连续切片；厚切片能在相对较短的曝光时间内产生强烈的放射自显影图像。放射自显影片显示，DNA合成发生在大核复制带后区的一个狭窄部分。大核DNA合成占据了分裂间期的很大一部分，而Styx sp.中的小核DNA合成发生在前期早期，此时大核DNA合成正处于末期阶段。
