Wallace J M, McNeilly A S
J Reprod Fertil. 1985 Mar;73(2):505-15. doi: 10.1530/jrf.0.0730505.
Administration of charcoal-treated bovine follicular fluid to Damline ewes twice daily (i.v.) from Days 1 to 11 of the luteal phase (Day 0 = oestrus) resulted in a delay in the onset of oestrous behaviour and a significant increase in ovulation rate following cloprostenol-induced luteolysis on Day 12. During follicular fluid treatment plasma levels of FSH in samples withdrawn just before injection of follicular fluid at 09:00 h (i.e. 16 h after previous injection of follicular fluid) were initially suppressed, but by Day 8 of treatment had returned to those of controls. However, the injection of follicular fluid at 09:00 h on Day 8 still caused a significant suppression of FSH as measured during a 6-h sampling period. Basal LH levels were higher throughout treatment due to a significant increase in amplitude and frequency of pulsatile secretion. After cloprostenol-induced luteal regression at the end of treatment on Day 12, plasma levels of FSH increased 4-fold over those of controls and remained higher until the preovulatory LH surge. While LH concentrations were initially higher relative to those of controls, there was no significant difference in the amount of LH released immediately before or during the preovulatory surge. These results suggest that the increase in ovulation rate observed during treatment with bovine follicular fluid is associated with the change in the pattern of gonadotrophin secretion in the luteal and follicular phases of the cycle.
在黄体期第1天至第11天（第0天=发情期），每天两次（静脉注射）向达姆林母羊注射经活性炭处理的牛卵泡液，结果导致发情行为开始延迟，并且在第12天氯前列醇诱导黄体溶解后排卵率显著增加。在卵泡液处理期间，于09:00时（即上次注射卵泡液后16小时）注射卵泡液前采集的样本中，促卵泡素（FSH）的血浆水平最初受到抑制，但到处理第8天时已恢复至对照水平。然而，在第8天09:00时注射卵泡液，在6小时采样期内测量时仍导致FSH显著抑制。由于脉冲式分泌的幅度和频率显著增加，整个处理过程中基础促黄体生成素（LH）水平较高。在第12天处理结束时氯前列醇诱导黄体退化后，FSH的血浆水平比对照增加了4倍，并一直保持较高水平直至排卵前LH峰出现。虽然LH浓度最初相对于对照较高，但在排卵前峰出现之前或期间立即释放的LH量没有显著差异。这些结果表明，在用牛卵泡液处理期间观察到的排卵率增加与周期黄体期和卵泡期促性腺激素分泌模式的变化有关。
