Department of Medicine, The University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA; Department of Surgery, The University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA.
Department of Medicine, The University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA; Department of Surgery, The University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA; Department of Pathology, The University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA.
STAR Protoc. 2024 Sep 20;5(3):103281. doi: 10.1016/j.xpro.2024.103281. Epub 2024 Aug 31.
Cancer cachexia mouse models are needed to recapitulate the clinical features of patients with cachexia. Here, we present a protocol for the establishment and evaluation of cancer cachexia mouse models. We delineate the steps in preparing tumor cells for inoculation and surgical procedures. After the establishment of these mouse models, we describe essential techniques to assess cancer cachexia, including grip strength evaluation, tissue collection, and the calculation of cross-sectional areas of muscle tissue. For complete details on the use and execution of this protocol, please refer to Liu et al., Yang et al., Shi et al., and Zhou et al..
需要建立癌症恶病质小鼠模型来重现恶病质患者的临床特征。在这里，我们提供了一种建立和评估癌症恶病质小鼠模型的方案。我们详细说明了为接种和手术准备肿瘤细胞的步骤。在建立这些小鼠模型后，我们描述了评估癌症恶病质的基本技术，包括握力评估、组织采集和肌肉组织横截面积的计算。如需详细了解本方案的使用和执行，请参考 Liu 等人、Yang 等人、Shi 等人和 Zhou 等人的研究。
