Department of Molecular and Medical Pharmacology, University of California, Los Angeles, Los Angeles, CA 90095, USA.
Department of Molecular and Medical Pharmacology, University of California, Los Angeles, Los Angeles, CA 90095, USA; Department of Urology, University of California, Los Angeles, Los Angeles, CA 90095, USA.
STAR Protoc. 2024 Sep 20;5(3):103239. doi: 10.1016/j.xpro.2024.103239. Epub 2024 Aug 2.
Recapitulating spontaneous metastasis in preclinical models is crucial for understanding mechanisms underlying cancer progression and testing effective therapeutic interventions. We present a protocol for establishing and characterizing the spontaneous metastasis model in mice. We describe steps for generating primary tumors, tumor resection, monitoring metastatic dissemination, and evaluating metastatic burden using histological and imaging techniques. This protocol provides a valuable tool for studying metastasis in vivo and testing therapeutic strategies aimed at preventing or targeting metastatic diseases. For complete details on the use and execution of this protocol, please refer to Liu et al..
重现临床前模型中的自发转移对于理解癌症进展的机制和测试有效的治疗干预措施至关重要。我们提出了一种在小鼠中建立和描述自发转移模型的方案。我们描述了生成原发性肿瘤、肿瘤切除、监测转移扩散以及使用组织学和成像技术评估转移负担的步骤。该方案为研究体内转移和测试旨在预防或靶向转移疾病的治疗策略提供了有价值的工具。有关该方案的使用和执行的完整详细信息，请参阅 Liu 等人的研究。
