血清和十四酰佛波醇-12-十四酸酯-13-乙酸酯对哺乳期小鼠卵巢细胞及器官培养中促卵泡激素刺激的4-雄烯-3,17-二酮转化为雌激素的影响。

Effect of serum and 12-O-tetradecanoyl-phorbol-13-acetate on FSH-stimulated conversion of 4-androstene-3,17-dione to oestrogens in cell and organ cultures of suckling mouse ovaries.

作者信息

Terada N, Kuroda H, Takatsuka D, Matsumoto K, Kitamura Y

出版信息

J Steroid Biochem. 1985 Mar;22(3):401-5. doi: 10.1016/0022-4731(85)90445-5.

Abstract

The effect of serum and 12-O-tetradecanoylphorbol-13-acetate (TPA) on the FSH-stimulated oestrogen production was studied in both cell and organ cultures. Ovaries were removed from (WB X C57BL/6)F1 mice at 9-days of age, and the conversion of 4-androstene-3,17 -dione to oestrogens was stimulated by the addition of FSH in vitro. Either 10% serum (fetal calf, mouse, rat and horse) or 0.1 microM TPA markedly inhibited the FSH-stimulated oestrogen production by dispersed and cultured ovarian cells. In contrast, neither serum nor TPA influenced the oestrogen production in the organ culture. This suggests that the presence of tissue architecture may prevent the inhibitory effect of serum and TPA on the FSH-stimulated oestrogen production.

摘要

在细胞培养和器官培养中研究了血清和12 - O - 十四烷酰佛波醇 - 13 - 乙酸酯（TPA）对促卵泡激素（FSH）刺激的雌激素生成的影响。在9日龄时从（WB×C57BL/6）F1小鼠中取出卵巢，在体外通过添加FSH刺激4 - 雄烯 - 3,17 - 二酮向雌激素的转化。10%血清（胎牛、小鼠、大鼠和马血清）或0.1微摩尔TPA均显著抑制分散培养的卵巢细胞中FSH刺激的雌激素生成。相比之下，血清和TPA均不影响器官培养中的雌激素生成。这表明组织结构的存在可能会阻止血清和TPA对FSH刺激的雌激素生成的抑制作用。