Terada N, Kuroda H, Namiki M, Kitamura Y, Matsumoto K
J Steroid Biochem. 1984 Mar;20(3):741-5. doi: 10.1016/0022-4731(84)90078-5.
The stimulative effect of FSH on aromatase activity was investigated in ovaries of fetal (on days 17 and 18 of gestation) and neonatal mice (on days 0, 3, 6 and 9 after birth). Two to six ovaries, were cultured for 48 h in 2 ml of Medium 199 supplemented with insulin (5 micrograms/ml) and [1 alpha, 2 alpha, 6 alpha, 7 alpha, beta-3H]4-androstene-3,17-dione (0.35 microM) in the presence or absence of porcine FSH (0.5 units/ml) and the amount of [3H]oestradiol-17 beta and [3H]oestrone produced was estimated. In the presence of FSH, aromatase activity per ovary, which was found in all fetal and neonatal ovaries examined, increased with age. In the absence of FSH, however, the production of oestrogens could be demonstrated only in ovaries from 3- to 9-day old mice. FSH increased the aromatase activity by up to 10-fold. In spite of the stimulative effect of FSH on aromatase activity, FSH exerted no significant effect on DNA synthesis of the ovaries. The formation of primordial follicles could not be observed histologically in ovaries of fetal mice on day 17 of gestation, although the ovaries of 6- and 9-day old mice contained multilayered follicles. These results show that FSH stimulates the aromatase activity of the mouse ovary even before the formation of primordial follicles and that the stimulative effect of FSH on ovarian aromatase is not due to the proliferation of ovarian cells.
在胎鼠（妊娠第17和18天）及新生小鼠（出生后第0、3、6和9天）的卵巢中研究了促卵泡激素（FSH）对芳香化酶活性的刺激作用。将两到六个卵巢在含有胰岛素（5微克/毫升）和[1α,2α,6α,7α,β - 3H]4 - 雄烯 - 3,17 - 二酮（0.35微摩尔）的2毫升199培养基中培养48小时，培养过程中分别加入或不加入猪促卵泡激素（0.5单位/毫升），然后测定生成的[3H]雌二醇 - 17β和[3H]雌酮的量。在促卵泡激素存在的情况下，在所检测的所有胎鼠和新生小鼠卵巢中均发现每个卵巢的芳香化酶活性随年龄增加。然而，在没有促卵泡激素的情况下，仅在3至9日龄小鼠的卵巢中能检测到雌激素的生成。促卵泡激素可使芳香化酶活性增加高达10倍。尽管促卵泡激素对芳香化酶活性有刺激作用，但它对卵巢的DNA合成没有显著影响。在妊娠第17天的胎鼠卵巢中，组织学上未观察到原始卵泡的形成，尽管6日龄和9日龄小鼠的卵巢中含有多层卵泡。这些结果表明，促卵泡激素在原始卵泡形成之前就能刺激小鼠卵巢的芳香化酶活性，并且促卵泡激素对卵巢芳香化酶的刺激作用并非由于卵巢细胞的增殖。
