College of Chemistry and Material Science Shandong Agricultural University/Key Laboratory of Agricultural Film Application of Ministry of Agriculture and Rural Affairs, Tai'an, Shandong 271018, PR China; Sanya Nanfan Research Institute of Hainan University, National Key Laboratory for Tropical Crop Breeding, Key Laboratory of Biotechnology of Salt Tolerant Crops of Hainan Province, School of Nanfan, School of Tropical Agriculture and Forestry, Hainan University, Hainan province, PR China.
College of Chemistry and Material Science Shandong Agricultural University/Key Laboratory of Agricultural Film Application of Ministry of Agriculture and Rural Affairs, Tai'an, Shandong 271018, PR China.
Ecotoxicol Environ Saf. 2024 Sep 15;283:116958. doi: 10.1016/j.ecoenv.2024.116958. Epub 2024 Aug 31.
Apple replant disease (ARD) negatively affects plant growth and reduces yields in replanted orchards. In this study, biochar and humic acid were applied to apple replant soil. We aimed to investigate whether biochar and humic acid could promote plant growth and alleviate apple replant disease by reducing the growth of harmful soil microorganisms, changing soil microbial community structure, and improving the soil environment. This experiment included five treatments: apple replant soil (CK), apple replant soil with methyl bromide fumigation (FM), replant soil with biochar addition (2 %), replant soil with humic acid addition (1.5 ‰), and replant soil with biochar combined with humic acid. Seedling biomass, the activity of antioxidant enzymes in the leaves and roots, and soil environmental variables were measured. Microbial community composition and structure were analyzed using ITS gene sequencing. Biochar and humic acid significantly reduced the abundance of Fusarium and promoted the recovery of replant soil microbial communities. Biochar and humic acid also increased the soil enzymes activity (urease, invertase, neutral phosphatase, and catalase), the plant height, fresh weight, dry weight, the activity of antioxidant enzymes (superoxide dismutase, peroxidase, and catalase), and root indexes of apple seedlings increased in replant soil. In sum, We can use biochar combined with humic acid to alleviate apple replant disease.
苹果再植病（ARD）会影响植株生长，降低再植果园的产量。本研究将生物炭和腐植酸应用于苹果再植土壤中，旨在探讨生物炭和腐植酸能否通过减少有害土壤微生物的生长、改变土壤微生物群落结构和改善土壤环境来促进植物生长和缓解苹果再植病。本试验包括五个处理：苹果再植土壤（CK）、苹果再植土壤用溴甲烷熏蒸（FM）、添加生物炭的再植土壤（2%）、添加腐植酸的再植土壤（1.5‰）和添加生物炭和腐植酸的再植土壤。测定了幼苗生物量、叶片和根系抗氧化酶活性以及土壤环境变量。采用 ITS 基因测序分析微生物群落组成和结构。生物炭和腐植酸显著降低了镰刀菌的丰度，促进了再植土壤微生物群落的恢复。生物炭和腐植酸还增加了土壤酶活性（脲酶、转化酶、中性磷酸酶和过氧化氢酶），提高了苹果幼苗在再植土壤中的株高、鲜重、干重、抗氧化酶活性（超氧化物歧化酶、过氧化物酶和过氧化氢酶）和根系指标。总之，我们可以使用生物炭与腐植酸联合缓解苹果再植病。
