State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, PR China.
Center for Wetland Conservation and Research, Hengshui University, Hengshui, 053000, Hebei Province, PR China; Collaborative Innovation Center for Wetland Conservation and Green Development of Hebei Province, Hengshui, 053000, PR China.
Insect Biochem Mol Biol. 2024 Oct;173:104180. doi: 10.1016/j.ibmb.2024.104180. Epub 2024 Aug 30.
Winged parthenogenetic aphids are mainly responsible for migration and dispersal. Aphid alarm pheromone (E)-β-Farnesene (EBF) has dual effects on repelling and stimulating wing differentiation in aphids. Previous studies have shown that the odorant coreceptor SmisOrco is involved in the perception of EBF by S. miscanthi; however, its EBF-specific odorant receptor (OR) and the difference between winged and wingless aphids remain unclear. In this study, the Xenopus oocyte expression system and RNAi technology were used to detect the transmission of EBF signals, and it was found that the olfactory receptor SmisOR5 is an EBF-specific OR in S. miscanthi and is specifically highly expressed in the antennae of winged aphids. Furthermore, when OR5 was silenced with dsRNA, the repellent effect of EBF was weakened, and aphids showed more active aimless movements. Therefore, as a specific OR for EBF, the high expression level of SmisOR5 in winged aphids suggests a molecular basis for its high sensitivity to EBF. This study advances our understanding of the molecular mechanisms of aphid EBF perception and provides novel ideas for effective management and prevention of the migration of winged aphids.
有翅型孤雌生殖蚜虫主要负责迁移和扩散。蚜虫报警信息素（E）-β-法呢烯（EBF）对蚜虫的翅分化有驱避和刺激的双重作用。先前的研究表明，气味受体核心 SmisOrco 参与了长芒野燕麦对 EBF 的感知；然而，其 EBF 特异性气味受体（OR）以及有翅和无翅蚜虫之间的差异仍不清楚。在这项研究中，使用非洲爪蟾卵母细胞表达系统和 RNAi 技术检测了 EBF 信号的传递，结果发现嗅觉受体 SmisOR5 是长芒野燕麦中 EBF 的特异性 OR，并且特异性地在有翅蚜虫的触角中高度表达。此外，当 OR5 被 dsRNA 沉默时，EBF 的驱避作用减弱，蚜虫表现出更活跃的漫无目的的运动。因此，作为 EBF 的特异性 OR，SmisOR5 在有翅蚜虫中的高表达水平表明了其对 EBF 高敏感性的分子基础。本研究推进了我们对蚜虫 EBF 感知的分子机制的理解，并为有效管理和预防有翅蚜虫的迁移提供了新的思路。
