Abdoul-Latif Fatouma Mohamed, El Mhamdi My Ismail, Ainane Ayoub, Ali Ali Merito, Oumaskour Khadija, Cherroud Sanaa, Cacciatore Stefano, Ainane Tarik
Medicinal Research Institute, Centre d'Etudes et de Recherche de Djibouti, IRM-CERD, Route de l'Aéroport, Haramous B.P. 486, Djibouti City 77101, Djibouti.
Superior School of Technology of Khenifra (EST-Khenifra), University of Sultan Moulay Slimane, BP 170, Khenifra 54000, Morocco.
Molecules. 2025 Apr 4;30(7):1621. doi: 10.3390/molecules30071621.
This work aims to develop an insecticidal biofilm based on Calothrixin A, collagen, and chitosan for the protection of pea seeds. The main objective is to improve the ingredient concentrations maximizing the insecticidal activity of the biofilm and to study the desorption of Calothrixin A according to the diffusion parameters. Eight biofilm formulations were prepared with different concentrations of the components and tested on and . The results show that a high concentration of Calothrixin A tended to increase insecticidal activity, although this increase was not always significant, while a higher concentration of collagen and chitosan reduced insecticidal activity, probably by limiting the diffusion of the active ingredient. The prediction models for insecticidal activity showed that the interaction of the factors had no significant impact on the responses, but the model for presented better accuracy. The diffusion tests revealed that the CB3C-5 biofilm, with high diffusion parameters, correlated with insecticidal activity. The characterization of the CB3C-5 biofilm showed adequate physical, mechanical, thermal, and structural properties for agricultural seed storage application. Moreover, the computational approach showed that Calothrixin A interacts more efficiently with the OR5-Orco complex than with the small OBP, disrupting the olfactory detection of insects. This mechanism highlights the targeting of the olfactory complex as a potential strategy to control insect pests. This research contributes to the understanding of the role of marine-based biofilms for seed protection and opens perspectives for the development of ecological solutions against insect pests, particularly in the field of sustainable agriculture.
这项工作旨在开发一种基于卡洛西菌素A、胶原蛋白和壳聚糖的杀虫生物膜，用于保护豌豆种子。主要目标是提高成分浓度，使生物膜的杀虫活性最大化，并根据扩散参数研究卡洛西菌素A的解吸情况。制备了八种不同成分浓度的生物膜配方，并在……上进行了测试。结果表明，高浓度的卡洛西菌素A往往会提高杀虫活性，尽管这种提高并不总是显著的，而较高浓度的胶原蛋白和壳聚糖则会降低杀虫活性，可能是通过限制活性成分的扩散。杀虫活性的预测模型表明，各因素之间的相互作用对响应没有显著影响，但……模型的准确性更高。扩散测试表明，具有高扩散参数的CB3C-5生物膜与杀虫活性相关。CB3C-5生物膜的表征显示出其具有适合农业种子储存应用的物理、机械、热和结构特性。此外，计算方法表明，卡洛西菌素A与OR5-Orco复合物的相互作用比与小OBP的相互作用更有效，从而破坏了昆虫的嗅觉检测。这一机制突出了将嗅觉复合物作为控制害虫的潜在策略。这项研究有助于理解海洋基生物膜在种子保护中的作用，并为开发针对害虫的生态解决方案开辟了前景，特别是在可持续农业领域。
