Chambers A F, Wilson S
Mol Cell Biol. 1985 Apr;5(4):728-33. doi: 10.1128/mcb.5.4.728-733.1985.
Studies on ts mutants of avian sarcoma viruses have previously implicated the src gene product (pp60src) kinase function in in vitro transformation. The role of src in vivo, however, has not been clearly defined. Using a sensitive and quantitative assay that was developed in chicken embryos (Chambers et al., Cancer Res. 42:4018-4025, 1982), we tested the in vivo tumorigenic properties of cells transformed with LA23, an avian sarcoma virus that is temperature sensitive for in vitro transformation. We found that the in vivo growth ability of these cells was temperature sensitive and that this in vivo behavior correlated with the in vitro transformation behavior (growth in soft agar and saturation density).
此前，针对禽肉瘤病毒的温度敏感（ts）突变体的研究表明，src基因产物（pp60src）的激酶功能与体外转化有关。然而，src在体内的作用尚未明确界定。我们利用在鸡胚中开发的一种灵敏且定量的检测方法（钱伯斯等人，《癌症研究》42:4018 - 4025，1982年），测试了用LA23转化的细胞的体内致瘤特性，LA23是一种对体外转化具有温度敏感性的禽肉瘤病毒。我们发现，这些细胞的体内生长能力具有温度敏感性，且这种体内行为与体外转化行为（在软琼脂中的生长和饱和密度）相关。
