用温度敏感型病毒src突变体转化的细胞在体内生长对温度敏感。
Cells transformed with a ts viral src mutant are temperature sensitive for in vivo growth.
作者信息
Chambers A F, Wilson S
出版信息
Mol Cell Biol. 1985 Apr;5(4):728-33. doi: 10.1128/mcb.5.4.728-733.1985.
Abstract
Studies on ts mutants of avian sarcoma viruses have previously implicated the src gene product (pp60src) kinase function in in vitro transformation. The role of src in vivo, however, has not been clearly defined. Using a sensitive and quantitative assay that was developed in chicken embryos (Chambers et al., Cancer Res. 42:4018-4025, 1982), we tested the in vivo tumorigenic properties of cells transformed with LA23, an avian sarcoma virus that is temperature sensitive for in vitro transformation. We found that the in vivo growth ability of these cells was temperature sensitive and that this in vivo behavior correlated with the in vitro transformation behavior (growth in soft agar and saturation density).
摘要
此前，针对禽肉瘤病毒的温度敏感（ts）突变体的研究表明，src基因产物（pp60src）的激酶功能与体外转化有关。然而，src在体内的作用尚未明确界定。我们利用在鸡胚中开发的一种灵敏且定量的检测方法（钱伯斯等人，《癌症研究》42:4018 - 4025，1982年），测试了用LA23转化的细胞的体内致瘤特性，LA23是一种对体外转化具有温度敏感性的禽肉瘤病毒。我们发现，这些细胞的体内生长能力具有温度敏感性，且这种体内行为与体外转化行为（在软琼脂中的生长和饱和密度）相关。
相似文献
1
Cells transformed with a ts viral src mutant are temperature sensitive for in vivo growth.用温度敏感型病毒src突变体转化的细胞在体内生长对温度敏感。
Mol Cell Biol. 1985 Apr;5(4):728-33. doi: 10.1128/mcb.5.4.728-733.1985.
2
A host-dependent temperature-sensitive mutant of Rous sarcoma virus: evidence for host factors affecting transformation.劳斯肉瘤病毒的一种宿主依赖性温度敏感突变体：影响转化的宿主因子的证据。
Virology. 1988 Oct;166(2):561-72. doi: 10.1016/0042-6822(88)90527-2.
3
Mutations in v-Src SH3 and catalytic domains that jointly confer temperature-sensitive transformation with minimal temperature-dependent changes in cellular tyrosine phosphorylation.v-Src SH3结构域和催化结构域中的突变共同赋予温度敏感性转化，同时细胞酪氨酸磷酸化的温度依赖性变化最小。
J Virol. 1994 Jul;68(7):4392-9. doi: 10.1128/JVI.68.7.4392-4399.1994.
4
A mutation in v-src that removes a single conserved residue in the SH-2 domain of pp60v-src restricts transformation in a host-dependent manner.v-src 中的一个突变会去除 pp60v-src 的 SH-2 结构域中的一个保守残基，这种突变以宿主依赖的方式限制转化。
J Virol. 1989 Jan;63(1):338-48. doi: 10.1128/JVI.63.1.338-348.1989.
5
Rous sarcoma virus variants that encode src proteins with an altered carboxy terminus are defective for cellular transformation.编码具有改变的羧基末端的src蛋白的劳氏肉瘤病毒变体在细胞转化方面存在缺陷。
J Virol. 1985 Aug;55(2):314-21. doi: 10.1128/JVI.55.2.314-321.1985.
6
Inhibition by quercetin of the release of density dependent-inhibition of cell growth in RSV-transformed chicken cells.
Cell Biol Int Rep. 1984 Nov;8(11):939-47. doi: 10.1016/0309-1651(84)90192-9.
7
Biological properties of "partial" transformation mutants of Rous sarcoma virus and characterization of their pp60src kinase.劳氏肉瘤病毒“部分”转化突变体的生物学特性及其pp60src激酶的表征
J Virol. 1981 Jan;37(1):445-58. doi: 10.1128/JVI.37.1.445-458.1981.
8
Lack of correlation between pp60src kinase activity and transformation parameters in cells infected with temperature-conditional mutants of Rous sarcoma virus.劳氏肉瘤病毒温度条件突变体感染的细胞中，pp60src激酶活性与转化参数之间缺乏相关性。
J Gen Virol. 1981 Feb;52(Pt 2):395-9. doi: 10.1099/0022-1317-52-2-395.
9
A ts T mutant of Schmidt Ruppin strain of Rous sarcoma virus restricted at 39.5 degrees C for the morphological transformation and the tumorigenicity of chicken embryo fibroblasts.劳氏肉瘤病毒施密特-鲁平株的一种ts T突变体在39.5摄氏度时，鸡胚成纤维细胞的形态转化和致瘤性受到限制。
Int J Cancer. 1982 Jan 15;29(1):69-76. doi: 10.1002/ijc.2910290112.
10
Site-directed mutagenesis of the src gene of Rous sarcoma virus: construction and characterization of a deletion mutant temperature sensitive for transformation.劳氏肉瘤病毒src基因的定点诱变：对转化温度敏感的缺失突变体的构建与特性分析
J Virol. 1982 Nov;44(2):683-91. doi: 10.1128/JVI.44.2.683-691.1982.
引用本文的文献
1
Quantitative genetic analysis of tumor progression.肿瘤进展的数量遗传学分析
Cancer Metastasis Rev. 1985;4(2):173-92. doi: 10.1007/BF00050694.
2
Use of NeoR B16F1 murine melanoma cells to assess clonality of experimental metastases in the immune-deficient chick embryo.
Clin Exp Metastasis. 1988 Mar-Apr;6(2):171-82. doi: 10.1007/BF01784847.
3
Molecular determinants of metastatic transformation.转移转化的分子决定因素。
Environ Health Perspect. 1991 Jun;93:91-5. doi: 10.1289/ehp.919391.
4
Experimental metastasis of oncogene-transformed NIH 3T3 cells in chick embryo.致癌基因转化的NIH 3T3细胞在鸡胚中的实验性转移。
Jpn J Cancer Res. 1992 Mar;83(3):274-80. doi: 10.1111/j.1349-7006.1992.tb00100.x.
本文引用的文献
1
Pseudoneoplastic nonspecific proliferative lesions on the chorio-allantois of the chick embryo.鸡胚绒毛尿囊膜上的假性肿瘤性非特异性增殖性病变。
Br J Cancer. 1955 Mar;9(1):222-8. doi: 10.1038/bjc.1955.18.
2
INVASION AND METASTASIS OF HETEROLOGOUS TUMORS IN THE CHICK EMBRYO.异种肿瘤在鸡胚中的侵袭与转移
Prog Exp Tumor Res. 1964;4:98-125. doi: 10.1159/000385975.
3
Animal viruses and embryos.动物病毒与胚胎
Q Rev Biol. 1960 Dec;35:261-312. doi: 10.1086/403189.
4
The role of a natural antibody in the rejection of mouse tumor cells by the chick embryo.天然抗体在鸡胚排斥小鼠肿瘤细胞中的作用。
J Exp Med. 1957 Jul 1;106(1):111-26. doi: 10.1084/jem.106.1.111.
5
Tumor transplantation to the chick embryo.肿瘤移植到鸡胚
Ann N Y Acad Sci. 1952 Aug 8;55(2):313-29. doi: 10.1111/j.1749-6632.1952.tb26547.x.
6
A model system for studying metastasis using the embryonic chick.一种利用鸡胚研究转移的模型系统。
Cancer Res. 1982 Oct;42(10):4018-25.
7
Transepithelial invasion and intramesenchymal infiltration of the chick embryo chorioallantois by tumor cell lines.肿瘤细胞系对鸡胚尿囊膜的经上皮侵袭和间质内浸润。
Cancer Res. 1982 May;42(5):1826-37.
8
Temperature-sensitive tumorigenicity of cells transformed by a mutant of Moloney sarcoma virus.由莫洛尼肉瘤病毒突变体转化的细胞的温度敏感性致瘤性
Proc Natl Acad Sci U S A. 1980 Mar;77(3):1501-5. doi: 10.1073/pnas.77.3.1501.
9
Oncogenes of spontaneous and chemically induced tumors.自发肿瘤和化学诱导肿瘤的癌基因。
Adv Cancer Res. 1982;36:149-63. doi: 10.1016/s0065-230x(08)60424-8.
10
Selection for experimental metastatic ability of heterologous tumor cells in the chick embryo after DNA-mediated transfer.
Cancer Res. 1984 Sep;44(9):3970-5.
Zotero 插件