Armstrong P B, Quigley J P, Sidebottom E
Cancer Res. 1982 May;42(5):1826-37.
The ability of cultured tumor cell lines to invade across epithelia was studied by placing 10(4) to 10(6) dispersed cells on the chorionic epithelium (CE) of the chorioallantoic membrane of the 10-day chick embryo. Tumor lines included Walker carcinosarcoma, F87 cl 6T2 and B16-BL6 melanomas, and KiSV-NIH, 3B77SC4, and HT 1080 sarcomas. The CE is a bilayer of cells with a superficial periderm overlying a basal layer. Invasion across an intact CE was very weak (limited to the formation of "microtumors" by a small fraction of the inoculated cells in 5 to 50% of the embryos) but was massive (most or all of the inoculated cells invaded in over 99% of the embryos) if the chorioallantoic membrane was traumatized in a fashion which disrupted the periderm but left the basal layer intact. Normal fibroblasts also invaded across the traumatized CE. The histological picture of invasion suggests that cells inoculated on the traumatized CE induced large-scale active retraction of the basal layer, resulting in the formation of large gaps in its continuity. Migration into the subjacent mesoderm occurred through these gaps. The nodules formed by both tumorigenic and normal cells became extensively vascularized within 3 days.
通过将10⁴至10⁶个分散的细胞置于10日龄鸡胚绒毛尿囊膜的绒毛膜上皮（CE）上，研究了培养的肿瘤细胞系穿越上皮的侵袭能力。肿瘤细胞系包括沃克癌肉瘤、F87 cl 6T2和B16 - BL6黑色素瘤，以及KiSV - NIH、3B77SC4和HT 1080肉瘤。CE是一层由表层的周皮覆盖在基底层之上的双层细胞。穿过完整CE的侵袭非常微弱（在5%至50%的胚胎中，只有一小部分接种细胞形成“微肿瘤”），但如果以破坏周皮但使基底层保持完整的方式对绒毛尿囊膜造成创伤，侵袭则会大量发生（在超过99%的胚胎中，大部分或所有接种细胞都会侵袭）。正常成纤维细胞也会穿过受创伤的CE。侵袭的组织学图像表明，接种在受创伤CE上的细胞会诱导基底层大规模主动回缩，导致其连续性出现大的间隙。通过这些间隙迁移到下方的中胚层。致瘤细胞和正常细胞形成的结节在3天内会广泛血管化。
