College of Chemistry, Chemical Engineering and Resource Utilization, Northeast Forestry University, Harbin, 150040, China; Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin, 150040, China.
Key Laboratory of Soybean Molecular Design Breeding, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Harbin, 150040, China.
Chemosphere. 2024 Sep;364:143229. doi: 10.1016/j.chemosphere.2024.143229. Epub 2024 Aug 30.
Di(2-ethylhexyl)phthalate (DEHP) is one of the most widely used plasticizers in plastic manufacturing. However, the toxicological effects of DEHP on dandelions remain poorly understood. This study comprehensively analyzed and explored the response mechanisms of dandelions to 1, 10, 50, and 100 mg L DEHP influencing the morphophysiological growth, metabolomics, and molecular docking. DEHP reduced chlorophyll synthesis, inhibited plant growth, and induced oxidative-state-associated stress, which was manifested by the excessive production of reactive oxygen species, an increase in antioxidant enzyme activities, and enhanced synthesis of some osmoregulatory compounds, including proline and soluble protein. An analysis of the integrated biological response index showed that the toxicity was dose-dependent. Molecular docking demonstrated that DEHP could bind stably to three enzymes, and the binding energy was peroxidase (POD) > catalase (CAT) > superoxide dismutase (SOD). Metabolomics revealed that metabolite abundance and metabolic pathways were altered by DEHP, with 88 and 72 primary metabolites identified in shoots and roots, respectively. Amino acid, sugar, and organic acid metabolism were severely disturbed, with the most significant effects being on carbohydrate metabolism, valine, leucine, and isoleucine biosynthesis. Our study elucidated the influence of DEHP exposure on dandelions, providing new insights into the toxicity mechanisms and toxicological risk assessment.
邻苯二甲酸二（2-乙基己基）酯（DEHP）是塑料制造中使用最广泛的增塑剂之一。然而，DEHP 对蒲公英的毒理学影响仍知之甚少。本研究综合分析和探讨了 DEHP 对蒲公英的影响，浓度分别为 1、10、50 和 100mg/L，研究涉及形态生理生长、代谢组学和分子对接。DEHP 减少了叶绿素的合成，抑制了植物的生长，并诱导了氧化应激相关的应激，表现为活性氧的过度产生、抗氧化酶活性的增加和一些渗透调节化合物如脯氨酸和可溶性蛋白的合成增强。综合生物响应指数分析表明，毒性是剂量依赖性的。分子对接表明 DEHP 可以与三种酶稳定结合，结合能为过氧化物酶（POD）>过氧化氢酶（CAT）>超氧化物歧化酶（SOD）。代谢组学表明 DEHP 改变了代谢物丰度和代谢途径，在地上部和根部分别鉴定出 88 和 72 种主要代谢物。氨基酸、糖和有机酸代谢受到严重干扰，碳水化合物代谢、缬氨酸、亮氨酸和异亮氨酸生物合成受到的影响最大。我们的研究阐明了 DEHP 暴露对蒲公英的影响，为毒性机制和毒理学风险评估提供了新的见解。
