College of Resources and Environment, Key Laboratory of Agricultural Environment, Shandong Agricultural University, Tai'an 271000, PR China.
Department of Anesthesiology, Tai'an City Central Hospital, Tai'an 271000, PR China.
J Hazard Mater. 2023 Apr 5;447:130816. doi: 10.1016/j.jhazmat.2023.130816. Epub 2023 Jan 18.
Di(2-ethylhexyl)phthalate esters (DEHP) has attracted widespread attention due to its ecotoxicological effects on organisms. In this study, wheat seedlings were exposed to DEHP- contaminated soil with 4 concentration gradients (0, 1, 10, and 100 mg kg, respectively) for 30 days. The growth index, physiological index, oxidative damage system, and gene expression of wheat seedlings were comprehensively measured and analyzed. The results revealed that DEHP could reduce the germination rate of wheat. Only the 100 mg kg treatment group significantly inhibited root length, but no effect on plant height. At the biochemical level, photosynthetic pigments of wheat seedlings were promoted first and then inhibited, while the soluble sugar content presented a trend of "inhibition - activation - inhibition". The antioxidant enzymes (SOD and POD) presented an approximate parabolic trend, while it was opposite for CAT. Whereas the corresponding antioxidant enzyme genes were up-regulated, and the Hsp70 heat-shock protein gene was down-regulated. Finally, integrated biological response index (IBR) analysis showed that the DEHP toxicity to wheat seedlings was dose dependent. Molecular docking indicated that DEHP could stably bind to GBSS and GST by intermolecular force. Overall, this study provided constructive insights for a comprehensive assessment of the toxicity risk of DEHP to wheat.
邻苯二甲酸二（2-乙基己基）酯（DEHP）因其对生物的生态毒性效应而受到广泛关注。本研究采用浓度分别为 0、1、10 和 100mg/kg 的 DEHP 污染土壤对小麦幼苗进行了 30 天的暴露实验，综合测定和分析了小麦幼苗的生长指标、生理指标、氧化损伤系统和基因表达。结果表明，DEHP 会降低小麦的发芽率，仅 100mg/kg 处理组显著抑制了根长，但对株高没有影响。在生化水平上，小麦幼苗的光合色素先促进后抑制，而可溶性糖含量呈“抑制-激活-抑制”的趋势。抗氧化酶（SOD 和 POD）呈近似抛物线趋势，而 CAT 则相反。相应的抗氧化酶基因上调，Hsp70 热休克蛋白基因下调。最后，综合生物响应指数（IBR）分析表明，DEHP 对小麦幼苗的毒性具有剂量依赖性。分子对接表明 DEHP 可以通过分子间作用力稳定地与 GBSS 和 GST 结合。总之，该研究为全面评估 DEHP 对小麦的毒性风险提供了有价值的信息。
