Faculty of Medicine, Universidade Federal do Ceará, Fortaleza, Brazil.
College of Dentistry, University of Florida, Gainesville, Florida, USA.
Biofouling. 2024 Oct;40(9):593-601. doi: 10.1080/08927014.2024.2396013. Epub 2024 Sep 1.
invasive candidiasis is considered a global health problem. In such cases, biofilm formation on implanted devices represents a therapeutic challenge and the presence of metabolically inactive persistent cells (PCs) in these communities increases their tolerance to fungicidal drugs. This study investigated the influence of amoxicillin, AMX; cefepime, CEF; gentamicin, GEN; amikacin, AMK; vancomycin, VAN; and ciprofloxacin, CIP; on the production of PCs in biofilms of bloodstream isolates. 48 h-mature biofilms ( = 6) grown in RPMI-1640 supplemented with antibiotics were treated with 100 μg ml amphotericin B and then evaluated for PCs. Biofilms grown in the presence of antibiotics produced more PCs, up to 10×, when exposed to AMX and CIP; 5 × to CEF; and 6 × to GEN and VAN. The results indicate that antibiotics can modulate PC production in biofilms. This scenario may have clinical repercussions in immunocompromised patients under broad-spectrum antibiotic therapy.
侵袭性念珠菌病被认为是一个全球性的健康问题。在这种情况下，植入设备上生物膜的形成代表了一个治疗挑战，而这些群落中代谢不活跃的持久细胞 (PCs) 的存在增加了它们对杀真菌药物的耐受性。本研究调查了阿莫西林 (AMX)、头孢吡肟 (CEF)、庆大霉素 (GEN)、阿米卡星 (AMK)、万古霉素 (VAN) 和环丙沙星 (CIP) 对血流感染分离株生物膜中 PCs 产生的影响。在含有抗生素的 RPMI-1640 中生长 48 小时成熟的生物膜 ( = 6) 用 100 μg ml 两性霉素 B 处理，然后评估 PCs。当暴露于 AMX 和 CIP 时，生物膜中产生的抗生素可使 PCs 增加多达 10 倍；暴露于 CEF 时增加 5 倍；暴露于 GEN 和 VAN 时增加 6 倍。结果表明，抗生素可以调节生物膜中 PCs 的产生。这种情况可能在接受广谱抗生素治疗的免疫功能低下患者中产生临床影响。
