Childs G V
Peptides. 1985 Jan-Feb;6(1):103-7. doi: 10.1016/0196-9781(85)90084-1.
Stimulation of gonadotropes following castration or ovariectomy results in a shift in the gonadotrope population to cells that are mostly multihormonal. The purpose of these studies was to test this phenomenon, in vitro with the use of doublestains for LH and FSH applied to GnRH-stimulated gonadotropes. One-3 day monolayers were stimulated for 10 min-4 hr with 0.1 nM [D-Lys6] GnRH and then fixed and stained for both gonadotropins. After 60 min of stimulation, there was a significant increase in the proportion of gonadotropes that contained both hormones (from 57% to 74%) with a corresponding decrease in the proportion of cells that contained only one gonadotropin. There was no significant increase in the overall percentage of gonadotropes in the cell population indicating that the shift had probably occurred as a result of stimulation of the monohormonal gonadotropes to produce the other hormone. In addition, some of the stimulated gonadotropes showed the development of processes, some of which stained for only one of the gonadotropins. These data suggest that most gonadotropes may have the capacity to produce and store both hormones but they may perform these functions in separate regions of the same cell.
阉割或卵巢切除术后对促性腺激素细胞的刺激会导致促性腺激素细胞群体向大多为多激素分泌的细胞转变。这些研究的目的是在体外使用针对促黄体生成素（LH）和促卵泡生成素（FSH）的双重染色法，应用于促性腺激素释放激素（GnRH）刺激的促性腺激素细胞，来验证这一现象。将1 - 3天的单层细胞用0.1 nM [D - Lys6] GnRH刺激10分钟至4小时，然后固定并对两种促性腺激素进行染色。刺激60分钟后，同时含有两种激素的促性腺激素细胞比例显著增加（从57%增至74%），而仅含有一种促性腺激素的细胞比例相应下降。细胞群体中促性腺激素细胞的总体百分比没有显著增加，这表明这种转变可能是由于单激素分泌的促性腺激素细胞受到刺激而产生另一种激素所致。此外，一些受刺激的促性腺激素细胞显示出突起的形成，其中一些仅对一种促性腺激素染色。这些数据表明，大多数促性腺激素细胞可能有能力产生和储存两种激素，但它们可能在同一细胞的不同区域执行这些功能。
