Voĭvodov K I, Tysiachnaia I V, Gubnitskiĭ L S, Iakovleva V I, Berezin I V
Prikl Biokhim Mikrobiol. 1985 Mar-Apr;21(2):161-6.
The Citrobacter freundii 62 cells immobilized in PAAG and possessing the tyrosine-phenol-lyase (TPL) activity catalyse the synthesis of 3,4-dihydroxyphenyl-L-alanine (DOPA) from pyrocatechol and ammonium pyruvate. The synthesis of DOPA was studied using both free and immobilized bacterial cells. When the concentration of pyrocatechol is over 0.1 M the TPL activity of the cells is inhibited. The concentration of pyrocatechol can be increased up to 0.3 M by using an equimolar mixture of pyrocatechol and boric acid. The addition of ascorbic acid as an antioxidant results in a lower TPL activity of both free and immobilized bacterial cells.
固定在聚丙烯酰胺凝胶（PAAG）中且具有酪氨酸酚裂解酶（TPL）活性的弗氏柠檬酸杆菌62细胞催化由邻苯二酚和丙酮酸铵合成3,4-二羟基苯丙氨酸（DOPA）。使用游离和固定化细菌细胞研究了DOPA的合成。当邻苯二酚浓度超过0.1M时，细胞的TPL活性受到抑制。通过使用邻苯二酚和硼酸的等摩尔混合物，邻苯二酚的浓度可提高至0.3M。添加抗坏血酸作为抗氧化剂会导致游离和固定化细菌细胞的TPL活性降低。
