利用从草生欧文氏菌克隆的酪氨酸酚裂解酶基因在大肠杆菌中生产L-二羟基苯丙氨酸。
Production of L-dihydroxyphenylalanine in Escherichia coli with the tyrosine phenol-lyase gene cloned from Erwinia herbicola.
作者信息
Foor F, Morin N, Bostian K A
机构信息
Merck Research Laboratories, Rahway, New Jersey 07065.
出版信息
Appl Environ Microbiol. 1993 Sep;59(9):3070-5. doi: 10.1128/aem.59.9.3070-3075.1993.
Abstract
The gene (tutA) encoding tyrosine phenol-lyase from Erwinia herbicola was cloned into Escherichia coli, and fusions to the lac and tac promoters were constructed. The enzyme was expressed at high levels in E. coli in the presence of isopropyl-beta-D-thiogalactopyranoside or lactose as an inducer. L-Dihydroxyphenylalanine was synthesized in high yield from catechol, pyruvate, and ammonia by induced cells.
摘要
将来自草生欧文氏菌的编码酪氨酸酚裂解酶的基因（tutA）克隆到大肠杆菌中，并构建了与lac和tac启动子的融合体。在异丙基-β-D-硫代半乳糖苷或乳糖作为诱导剂存在的情况下，该酶在大肠杆菌中高水平表达。诱导细胞利用儿茶酚、丙酮酸和氨高产合成了L-二羟基苯丙氨酸。
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