Yeum Insun, Ko Donghwan, Lee Gyujin, Seok Hoik, Jung Byungjo
Department of Biomedical Engineering, Yonsei University, Wonju-Si, Republic of Korea.
Biomed Eng Lett. 2024 Jun 7;14(5):1125-1135. doi: 10.1007/s13534-024-00400-4. eCollection 2024 Sep.
Dual-mode optical imaging can simultaneously provide morphological and functional information. Furthermore, it can be integrated with projection mapping method to directly observe the images in the region of interest. This study was aimed to develop a dual-mode optical projection mapping system (DOPMS) that obtains laser speckle contrast image (LSCI) and subcutaneous vein image (SVI) and projects onto the region of interest, minimizing the spatial misalignment between the regions captured by the camera and projected by a projector. In in vitro and in vivo studies, LSCI and SVI were obtained and projected under single-mode illumination, where either the laser or light-emitting diode (LED) was activated, and under dual-mode illumination, where the laser and LED were activated simultaneously. In addition, fusion image (FI) of LSCI and SVI was implemented to selectively observe blood perfusion in the vein. DOPMS successfully obtained LSCI, SVI, and FI and projected them onto the identical region of interest, minimizing spatial misalignment. Single-mode illumination resulted in relatively clearer and noise-free images. Dual-mode illumination introduced speckle noise to SVI and FI but enabled real-time imaging by simultaneously employing LSCI, SVI, and FI. FI may be more effective for quasi-static evaluations before and after treatment under single-mode illumination and for real-time evaluation during treatment under dual-mode illumination owing to its faster image processing, albeit with a potential tradeoff in image quality.
双模光学成像能够同时提供形态学和功能信息。此外，它可以与投影映射方法相结合，直接观察感兴趣区域的图像。本研究旨在开发一种双模光学投影映射系统（DOPMS），该系统可获取激光散斑对比图像（LSCI）和皮下静脉图像（SVI），并将其投影到感兴趣区域，最大限度地减少相机捕获区域与投影仪投影区域之间的空间错位。在体外和体内研究中，分别在单模照明（即激活激光或发光二极管（LED））和双模照明（即同时激活激光和LED）条件下获取并投影LSCI和SVI。此外，还实现了LSCI和SVI的融合图像（FI），以选择性地观察静脉中的血液灌注情况。DOPMS成功获取了LSCI、SVI和FI，并将它们投影到相同的感兴趣区域，最大限度地减少了空间错位。单模照明产生的图像相对更清晰且无噪声。双模照明给SVI和FI引入了散斑噪声，但通过同时使用LSCI、SVI和FI实现了实时成像。FI可能在单模照明下治疗前后的准静态评估以及双模照明下治疗期间的实时评估中更有效，因为其图像处理速度更快，尽管在图像质量上可能存在潜在的权衡。
