Wang Qian-Wen, Zhu Jie-Wen, Gong Ling-Zhen
Department of Clinical Laboratory, Huangshi Central Hospital (Affiliated Hospital of Hubei Polytechnic University), Huangshi 435000, Hubei Province, China.
Hubei Key Laboratory of Kidney Disease Pathogenesis and Intervention, Huangshi 435000, Hubei Province, China.
World J Gastrointest Surg. 2024 Aug 27;16(8):2521-2527. doi: 10.4240/wjgs.v16.i8.2521.
Gastric cancer is one of the most common malignant tumors worldwide, and surgical resection is one of the main ways to treat gastric cancer. However, the immune status of postoperative patients is crucial for prognosis and survival, and immune cells play an important role in this process. Therefore, it is helpful to understand the immune status of postoperative patients by evaluating the levels of peripheral blood immune cells, especially total T cells (CD3+), helper T cells (CD3+CD4+), and suppressor T cells (CD3+CD8+), and its relationship to survival.
To analyzed the immune cells in peripheral blood of patients with gastric cancer after surgery, detect the levels of total T cells, helper T cells and suppressor T cells.
A total of 58 patients with gastric cancer who received surgical treatment were included in the retrospective study. Flow cytometry was used to detect the level of peripheral blood immune cells and analyze the correlation between total T cells, helper T cells and inhibitory T cells. To explore the relationship between these immune markers and patient survival.
The results showed that the levels of total T cells, helper T cells, and suppressor T cells changed in patients after gastric cancer surgery. There was a significant positive correlation between total T cells, helper T cells and suppressor T cells ( = 0.35, < 0.01; = 0.56, < 0.01). However, there was a negative correlation between helper T cells and suppressor T cells ( = -0.63, < 0.01). Follow-up showed that the survival rate of patients in the high-level total T cell group was significantly higher than that in the low-level group (28.87 ± 24.98 months 18.42 ± 16.21 months). The survival curve shows that the curve of patients in the high-level group is shifted to the upper right, and that of the low-level group is shifted downward. There was no significant difference between the levels of helper T cells and suppressor T cells and patient survival time.
By detecting peripheral blood immune cells with flow cytometry, we can initially evaluate the immune status of patients after gastric cancer surgery and initially explore its relationship with patient survival.
胃癌是全球最常见的恶性肿瘤之一，手术切除是治疗胃癌的主要方法之一。然而，术后患者的免疫状态对预后和生存至关重要，免疫细胞在此过程中发挥重要作用。因此，通过评估外周血免疫细胞水平，尤其是总T细胞（CD3 +）、辅助性T细胞（CD3 + CD4 +）和抑制性T细胞（CD3 + CD8 +）及其与生存的关系，有助于了解术后患者的免疫状态。
分析胃癌术后患者外周血中的免疫细胞，检测总T细胞、辅助性T细胞和抑制性T细胞水平。
本回顾性研究纳入了58例接受手术治疗的胃癌患者。采用流式细胞术检测外周血免疫细胞水平，并分析总T细胞、辅助性T细胞和抑制性T细胞之间的相关性。探讨这些免疫标志物与患者生存的关系。
结果显示，胃癌手术后患者的总T细胞、辅助性T细胞和抑制性T细胞水平发生了变化。总T细胞、辅助性T细胞和抑制性T细胞之间存在显著正相关（r = 0.35，P < 0.01；r = 0.56，P < 0.01）。然而，辅助性T细胞与抑制性T细胞之间存在负相关（r = -0.63，P < 0.01）。随访显示，总T细胞高水平组患者的生存率显著高于低水平组（28.87±24.98个月 vs 18.42±16.21个月）。生存曲线显示，高水平组患者的曲线向右上方移动，低水平组患者的曲线向下移动。辅助性T细胞和抑制性T细胞水平与患者生存时间之间无显著差异。
通过流式细胞术检测外周血免疫细胞，可初步评估胃癌术后患者的免疫状态，并初步探讨其与患者生存的关系。
