Liu Yue, Wang Zhiming, Li Ke, Song Ruihu, Xu Zhiqiang, Yu Tianhe, Wang Jing, Feng Xin, Chen Hao
State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, and College of Veterinary Medicine, Jilin University, Changchun 130062, China.
State Key Laboratory of Chemical Biology, Molecular Imaging Center, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China.
ACS Omega. 2024 Aug 19;9(34):36371-36379. doi: 10.1021/acsomega.4c03727. eCollection 2024 Aug 27.
As one of the research hotspots in recent years, gut microbiota have been proven to be closely related to host metabolism, nutrient absorption, and immune regulation. However, there are still many urgent issues in the research of gut microbiota, such as the localization and tracking of gut microbiota. In this research, two new fluorescent probes, EF and 6F, were developed by optimizing the structure of the positron salt small molecule probe F16. labeling experiments showed that EF and 6F can quickly label Gram-positive bacteria, and , as well as Gram-negative bacteria, and . Meanwhile, EF and 6F have little bacterial toxicity and are used at a maximum concentration of 200 μM. Compared with EF, 6F has better hydrophilicity and stronger fluorescence characteristics in aqueous solutions, making it more suitable for imaging within gut microbiota populations. The results of imaging experiments indicate that EF and 6F can label and image the intestinal microbiota colonized by the mouse intestinal mucosal epithelium without causing any damage to intestinal tissue. Compared with commercially available MitoTracker dyes and fluorescein 5-isothiocyanate (FITC) dyes, EF and 6F exhibit better biocompatibility. Therefore, the compounds EF and 6F synthesized in this study are novel small molecule probes suitable for imaging gut microbiota, providing a better probe selection for exploring complex gut microbiota.
作为近年来的研究热点之一，肠道微生物群已被证明与宿主代谢、营养吸收和免疫调节密切相关。然而，肠道微生物群的研究仍存在许多亟待解决的问题，如肠道微生物群的定位和追踪。在本研究中，通过优化正电子盐小分子探针F16的结构，开发了两种新型荧光探针EF和6F。标记实验表明，EF和6F能快速标记革兰氏阳性菌以及革兰氏阴性菌。同时，EF和6F的细菌毒性较小，最大使用浓度为200μM。与EF相比，6F具有更好的亲水性，在水溶液中具有更强的荧光特性，使其更适合在肠道微生物群中进行成像。成像实验结果表明，EF和6F能对定殖于小鼠肠道黏膜上皮的肠道微生物群进行标记和成像，且不会对肠道组织造成任何损伤。与市售的线粒体追踪染料和异硫氰酸荧光素（FITC）染料相比，EF和6F表现出更好的生物相容性。因此，本研究合成的化合物EF和6F是适用于肠道微生物群成像的新型小分子探针，为探索复杂的肠道微生物群提供了更好的探针选择。
