Wang Dongjing, Zeng Jiangyong, Wujin Cuomu, Ullah Qudrat, Su Zhonghua
Institute of Animal Husbandry and Veterinary, Tibet Autonomous Region Academy of Agricultural Sciences, Lhasa, Tibet, 850009, China.
Department of Theriogenology, Faculty of Veterinary and Animal Sciences, The University of Agriculture, Dera Ismail Khan, 29111, Pakistan.
Microb Pathog. 2024 Mar;188:106541. doi: 10.1016/j.micpath.2024.106541. Epub 2024 Jan 13.
Diarrhea is a prevalent health issue in farm animals and poses a significant challenge to the progress of animal husbandry. Recent evidence suggested that probiotics can alleviate diarrhea by maintaining gut microbial balance and enhancing the integrity of the intestinal barrier. However, there is a scarcity of studies investigating the efficacy of equine Lactobacillus reuteri in relieving E. coli-induced diarrhea. Hence, this study aimed to examine the potential of equine-derived Lactobacillus reuteri in alleviating E. coli diarrhea from the perspective of gut microbiota. Results demonstrated that supplementation of Lactobacillus reuteri had the potential to alleviate diarrhea induced by E. coli infection and restore the decline of tight junction genes, such as Claudin-1 and ZO-1. Additionally, Lactobacillus reuteri supplementation can restore the expression of inflammatory factors (IL-6, IL-10, TNF-α, and IFN-γ) and reduce colon inflammatory damage. Diversity analysis, based on amplicon sequencing, revealed a significant reduction in the diversity of gut microbiota during E. coli-induced diarrhea. Moreover, there were notable statistical differences in the composition and structure of gut microbiota among the different treatment groups. E. coli could induce gut microbial dysbiosis by decreasing the abundance of beneficial bacteria, including Lactobacillus, Bifidobacterium, Ligilactobacillus, Enterorhabdus, and Lachnospiraceae_UCG_001, in comparison to the control group. Conversely, supplementation with Lactobacillus reuteri could restore the abundance of beneficial bacteria and increase the diversity of the gut microbiota, thereby reshaping gut microbiota. Additionally, we also observed that supplementation with Lactobacillus reuteri alone improved the gut microbial composition and structure. In summary, the findings suggest that Lactobacillus reuteri can alleviate E. coli-induced diarrhea by preserving the integrity of the intestinal barrier and modulating the composition of the gut microbiota. These results not only contribute to understanding of the mechanism underlying the beneficial effects of Lactobacillus reuteri in relieving diarrhea, but also provide valuable insights for the development of probiotic products aimed at alleviating diarrheal diseases.
腹泻是家畜中普遍存在的健康问题，对畜牧业的发展构成重大挑战。最近的证据表明，益生菌可以通过维持肠道微生物平衡和增强肠道屏障的完整性来缓解腹泻。然而，关于马源罗伊氏乳杆菌缓解大肠杆菌诱导的腹泻功效的研究较少。因此，本研究旨在从肠道微生物群的角度研究马源罗伊氏乳杆菌缓解大肠杆菌腹泻的潜力。结果表明，补充罗伊氏乳杆菌有可能缓解大肠杆菌感染引起的腹泻，并恢复紧密连接基因（如Claudin-1和ZO-1）的下降。此外，补充罗伊氏乳杆菌可以恢复炎症因子（IL-6、IL-10、TNF-α和IFN-γ）的表达，并减少结肠炎症损伤。基于扩增子测序的多样性分析表明，大肠杆菌诱导腹泻期间肠道微生物群的多样性显著降低。此外，不同治疗组之间肠道微生物群的组成和结构存在显著统计学差异。与对照组相比，大肠杆菌可通过降低包括乳杆菌属、双歧杆菌属、 Ligilactobacillus、Enterorhabdus和毛螺菌科_UCG_001在内的有益菌丰度来诱导肠道微生物失调。相反，补充罗伊氏乳杆菌可以恢复有益菌的丰度，增加肠道微生物群的多样性，从而重塑肠道微生物群。此外，我们还观察到单独补充罗伊氏乳杆菌可改善肠道微生物组成和结构。总之，研究结果表明，罗伊氏乳杆菌可以通过保持肠道屏障的完整性和调节肠道微生物群的组成来缓解大肠杆菌诱导的腹泻。这些结果不仅有助于理解罗伊氏乳杆菌缓解腹泻有益作用的潜在机制，也为开发旨在缓解腹泻疾病的益生菌产品提供了有价值的见解。
