Jeong Suhui, Kim So Young, Myeong Hwain, Lim Eun-Kyung, An Sung-Min, Liang Huiling, Shrestha Krishna K, Uddin Md Salah, Kim Youngsuk, Yi Pyong-In, An Beum-Soo, Seo Sungbaek
Department of Biomaterials Science (BK21 FOUR Program), College of Natural Resources and Life Science/Life and Industry Convergence Research Institute, Pusan National University, Miryang 50463, Republic of Korea.
BioNanotechnology Research Center, KRIBB, 125 Gwahak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea.
ACS Omega. 2024 Aug 13;9(34):36531-36539. doi: 10.1021/acsomega.4c04523. eCollection 2024 Aug 27.
Natural polyphenols found in health supplements and drinks have antioxidant and anti-inflammatory properties. In particular, to determine the beneficial qualities of antioxidant drinks and beverages, consumers demand precise quantification of the total amount of polyphenols as on-site detection. Herein, we developed a new concept of portable beads suitable for the field detection available: colorimetric quantification of polyphenols equipped with color converting software applications in a smartphone or tablet PC. The yellowish beads contain ferric ions to react with polyphenol to produce blackish metal-phenolic complexes. It is simple to perform the detection procedure: dipping the beads in the analytical sample and out-taking a photo-converting into RGB color values and quantification of the existed polyphenol. The overall process was completed within 5 min. Compared with the Folin-Ciocalteu assay, which is a representative optical sensor kit for total phenolic content, the bead-based sensor showed a better limit of detection of 0.0415 mM for tannic acid and comparable sensing capability for a polyphenol-containing plant extract and brewed tea. The beads conserved the shape and sensitivity after months of storage or under environmental interference such as a change in the temperature.
健康补充剂和饮品中含有的天然多酚具有抗氧化和抗炎特性。特别是,为了确定抗氧化饮品和饮料的有益特性,消费者要求对多酚总量进行精确量化,以便进行现场检测。在此,我们开发了一种适用于现场检测的便携式珠子的新概念:配备智能手机或平板电脑中颜色转换软件应用程序的多酚比色定量法。淡黄色珠子含有铁离子,可与多酚反应生成黑色金属 - 酚类络合物。检测过程很简单:将珠子浸入分析样品中,拍照,转换为RGB颜色值,并对存在的多酚进行定量。整个过程在5分钟内完成。与用于总酚含量的代表性光学传感器试剂盒Folin - Ciocalteu测定法相比,基于珠子的传感器对单宁酸的检测限更低,为0.0415 mM,对含多酚的植物提取物和泡制茶叶具有相当的传感能力。经过数月储存或在温度变化等环境干扰下,珠子仍能保持形状和灵敏度。
ACS Omega. 2024-8-13
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