López-Fernández Olalla, Domínguez Rubén, Pateiro Mirian, Munekata Paulo E S, Rocchetti Gabriele, Lorenzo José M
Centro Tecnológico de la Carne de Galicia, Rúa Galicia No 4, Parque Tecnológico de Galicia, San Cibrao das Viñas, 32900 Ourense, Spain.
Department for Sustainable Food Process, Università Cattolica del Sacro Cuore, Via Emilia Parmense 84, 29122 Piacenza, Italy.
Antioxidants (Basel). 2020 Jun 2;9(6):479. doi: 10.3390/antiox9060479.
In recent years, the consumption of polyphenols has been increasing, largely due to its beneficial effects on health. They are present in a wide variety of foods, but their extraction and characterization are complicated since they are mostly in complex matrices. For this reason, the use of selective, sensitive, and versatile analytical techniques such as liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) is necessary. In this review, the most relevant studies of the last years regarding the analysis of polyphenols in different matrices by comprehensive LC-MS/MS are discussed. Relevant steps such as extraction, sample purification, and chromatographic analysis methods are emphasized. In particular, the following methodological aspects are discussed: (a) the proper selection of the extraction technique, (b) the extraction and elution solvents, (c) the purification step, (d) the selection of both stationary and mobile phases for the chromatographic separation of compounds, and (e) the different conditions for mass spectrometry. Overall, this review presents the data from the most recent studies, in a comprehensive way, thus providing and simplifying the information of the great variety of works that exist in the literature on this wide topic.
近年来，多酚的消费量一直在增加，这主要归功于其对健康的有益影响。它们存在于各种各样的食物中，但由于它们大多存在于复杂的基质中，其提取和表征过程较为复杂。因此，有必要使用选择性、灵敏且通用的分析技术，如液相色谱-串联质谱联用（LC-MS/MS）。在这篇综述中，讨论了近年来通过综合LC-MS/MS分析不同基质中多酚的最相关研究。重点强调了提取、样品净化和色谱分析方法等相关步骤。特别讨论了以下方法学方面：（a）提取技术的正确选择，（b）提取和洗脱溶剂，（c）净化步骤，（d）化合物色谱分离的固定相和流动相的选择，以及（e）质谱的不同条件。总体而言，本综述全面呈现了最新研究的数据，从而提供并简化了关于这个广泛主题的文献中存在的大量作品的信息。
