Kolipakala Rakshita Sukruth, Basu Suranjana, Sarkar Senjuti, Biju Beneta Merin, Salazar Daniela, Reddy Likhit, Pradeep Pushya, Yuvapriya Muniraj Krishnaveni, Nath Shrijita, Gall Riley, Samprathi Anish Hemanth, Balaji Harshitha, Koundinya Eeshaan A B, Shetye Aparna, Nagarajan Deepesh
Department of Biotechnology, M.S. Ramaiah University of Applied Sciences, Bangalore 560054, India.
Ecology and Genetics Research Unit, University of Oulu, Oulu 90014, Finland.
ACS Omega. 2024 Aug 7;9(34):36353-36370. doi: 10.1021/acsomega.4c03704. eCollection 2024 Aug 27.
Melanin is an amorphous, highly heterogeneous polymer found across all kingdoms of life. Although the properties of melanin can greatly vary, most forms are insoluble and strongly absorb light, appearing dark brown to black. Here, we describe a water-soluble form of melanin (peptidomelanin) secreted by the spores of (strain: melanoliber) during germination. Peptidomelanin is composed of an insoluble L-DOPA core polymer that is solubilized via short, copolymerized heterogeneous peptide chains forming a "corona" with a mean amino acid length of 2.6 ± 2.3. Based on experiments, we propose a biochemical copolymerization mechanism involving the hydroxylation of tyrosynylated peptides. Peptidomelanin is capable of chelating heavy metals such as lead, mercury, and uranium (as uranyl) in large quantities. Preliminary data indicates that peptidomelanin may have applications for the remediation of heavy metals , including in agricultural settings.
黑色素是一种无定形、高度异质的聚合物，存在于所有生命王国中。尽管黑色素的性质差异很大，但大多数形式都不溶于水且强烈吸收光线，呈现深棕色至黑色。在这里，我们描述了一种由（菌株：melanoliber）的孢子在萌发过程中分泌的水溶性黑色素形式（肽黑素）。肽黑素由不溶性的L-多巴核心聚合物组成，该聚合物通过短的、共聚的异质肽链溶解，形成平均氨基酸长度为2.6±2.3的“冠”。基于实验，我们提出了一种涉及酪氨酰化肽羟基化的生化共聚机制。肽黑素能够大量螯合铅、汞和铀（作为铀酰）等重金属。初步数据表明，肽黑素可能在重金属修复中具有应用价值，包括在农业环境中。
