Hong Hailong, Hu Yunhai, Shi Siyuan, Liu Ben, Zheng Wenya, Bo Ruonan, Xu Zhongjie, Wu Yifan, Cao Yu
College of Life Science and Resources and Environment, Yichun University, Yichun, China.
Jiangxi Lvke Agriculture and Animal Husbandry Technology Co., Ltd., Yichun, China.
Front Vet Sci. 2024 Aug 15;11:1413523. doi: 10.3389/fvets.2024.1413523. eCollection 2024.
Listeriosis is highly prevalent in the animal farming industry, with as the causative pathogen. To identify potential therapeutic targets for LM infection, we investigated the mechanisms of LM infection in goat uteri. We inoculated a group of goats with LM via jugular vein injection, isolated and raised them, and subsequently collected sterile samples of their uterine tissue after they exhibited clinical symptoms of LM infection. We used Giemsa staining, immunohistochemical staining, real-time qPCR, and Western blotting as experimental methods.First, we investigated the mechanism of Listeria monocytogenes (LM) infection in the goat uterus by examining the expression levels of listeriolysin O, E-cadherin, and tyrosine kinase c-Met in the uterus.Furthermore, we investigated the impact of LM infection on uterine autophagy and cell apoptosis. The results indicate that the injection of LM into the goats' jugular veins leads to LM infection in the goats' uteri. During LM survival inside the goat uterine cells, there is a significant increase in the expression levels of LLO, E-cadherin, and c-Met in the host uterine tissue. This suggests that LM may potentially infect goat uteri through the InlA/E-cadherin and InlB/c-Met pathways. Furthermore, LM infection increases the levels of apoptosis and autophagy in goat uteri. Apoptosis genes Bcl-2 and Bax, as well as autophagy-related genes LC3B, PINK1, and Parkin, exhibit varying degrees of changes in localization and expression in goat uteri, mediating the occurrence of apoptotic and autophagic responses.
李斯特菌病在畜牧业中非常普遍， 为致病病原体。为了确定针对单核细胞增生李斯特菌（LM）感染的潜在治疗靶点，我们研究了LM在山羊子宫内的感染机制。我们通过颈静脉注射对一组山羊接种LM，将它们隔离饲养，随后在它们出现LM感染的临床症状后收集其子宫组织的无菌样本。我们使用吉姆萨染色、免疫组织化学染色、实时定量聚合酶链反应（qPCR）和蛋白质免疫印迹法作为实验方法。首先，我们通过检测子宫中李斯特菌溶血素O、E-钙黏蛋白和酪氨酸激酶c-Met的表达水平，研究了山羊子宫中单核细胞增生李斯特菌（LM）的感染机制。此外，我们研究了LM感染对子宫自噬和细胞凋亡的影响。结果表明，向山羊颈静脉注射LM会导致山羊子宫发生LM感染。在LM在山羊子宫细胞内存活期间，宿主子宫组织中LLO、E-钙黏蛋白和c-Met的表达水平显著增加。这表明LM可能通过InlA/E-钙黏蛋白和InlB/c-Met途径感染山羊子宫。此外，LM感染会增加山羊子宫中的凋亡和自噬水平。凋亡基因Bcl-2和Bax以及自噬相关基因LC3B、PINK1和帕金蛋白在山羊子宫中的定位和表达呈现不同程度的变化，介导凋亡和自噬反应的发生。
