Grossini Elena, Venkatesan Sakthipryian, Pour Mohammad Mostafa Ola, Conti Andrea, Concina Diego, Opizzi Annalisa, Sanguedolce Agatino, Rinaldi Carmela, Russotto Sophia, Gramaglia Carla Maria, Zeppegno Patrizia, Panella Massimiliano
Laboratory of Physiology, Department of Translational Medicine, Università del Piemonte Orientale, 28100 Novara, Italy.
Department of Translational Medicine, Università del Piemonte Orientale, 28100 Novara, Italy.
Heliyon. 2024 Aug 8;10(16):e35850. doi: 10.1016/j.heliyon.2024.e35850. eCollection 2024 Aug 30.
It has recently been highlighted how a short healthy life-style program (LSP) can improve the functional outcomes of older people admitted to a Long-Term Care (LTC) facility. Although it is known that life-style medicine-based interventions can exert anti-aging effects through the modulation of oxidative stress and mitochondrial function, the mechanisms underlying the aforementioned effects have not been clarified, yet. For this reason, in this study, the outcomes were focused on the investigation of the possible mechanisms underlying the benefits of a short LSP in older people. This was achieved by examining circulating markers of oxidative stress and immunosenescence, such as Tymosin β (Tβ4), before and after LSP and the effects of plasma of older people undergone or not LSP on endothelial cells.
Fifty-four older people were divided into two groups (n = 27 each): subjects undergoing LSP and subjects not undergoing LSP (control). The LSP consisted of a combination of caloric restriction, physical activity, and psychological intervention and lasted 3 months. Plasma samples were taken before (T0) and after LSP (T1) and were used to measure thiobarbituric acid reactive substances (TBARS), 8-hydroxy-2-deoxyguanosine (8OHdG), 8-Isoprostanes (IsoP), glutathione (GSH), superoxide dismutase (SOD) activity and Tβ4. In addition, plasma was used to stimulate human vascular endothelial cells (HUVEC), which were examined for cell viability, mitochondrial membrane potential, reactive oxygen species (ROS) and mitochondrial ROS (MitoROS) release.
At T1, in LSP group we did not detect the increase of plasma TBARS and IsoP, which was observed in control. Also, plasma levels of 8OHdG were lower in LSP group vs control. In addition, LSP group only showed an increase of plasma GSH and SOD activity. Moreover, plasma levels of Tβ4 were more preserved in LSP group. Finally, at T1, in HUVEC treated with plasma from LSP group only we found an increase of the mitochondrial membrane potential and a reduction of ROS and MitoROS release in comparison with T0.
The results of this study showed that a short LSP in older persons exerts antiaging effects by modulating oxidative stress also at cellular levels. Implications of those findings could be related to both prognostic and therapeutic strategies, which could be pursued as antiaging methods.
最近有研究强调，短期健康生活方式计划（LSP）如何能改善入住长期护理（LTC）机构的老年人的功能结局。尽管已知基于生活方式医学的干预措施可通过调节氧化应激和线粒体功能发挥抗衰老作用，但上述作用的潜在机制尚未阐明。因此，在本研究中，重点关注短期LSP对老年人有益作用的潜在机制。这是通过检测LSP前后氧化应激和免疫衰老的循环标志物，如胸腺素β（Tβ4），以及接受或未接受LSP的老年人血浆对内皮细胞的影响来实现的。
54名老年人分为两组（每组n = 27）：接受LSP的受试者和未接受LSP的受试者（对照组）。LSP包括热量限制、体育活动和心理干预的组合，持续3个月。在LSP前（T0）和LSP后（T1）采集血浆样本，用于测量硫代巴比妥酸反应性物质（TBARS）、8-羟基-2-脱氧鸟苷（8OHdG）、8-异前列腺素（IsoP）、谷胱甘肽（GSH）、超氧化物歧化酶（SOD）活性和Tβ4。此外，用血浆刺激人血管内皮细胞（HUVEC），检测细胞活力、线粒体膜电位、活性氧（ROS）和线粒体ROS（MitoROS）释放。
在T1时，我们在LSP组中未检测到对照组中观察到的血浆TBARS和IsoP的增加。此外，LSP组的8OHdG血浆水平低于对照组。此外，LSP组仅显示血浆GSH和SOD活性增加。此外，LSP组中Tβ4的血浆水平得到更好的维持。最后，在T1时，与T0相比，仅用LSP组血浆处理的HUVEC中线粒体膜电位增加，ROS和MitoROS释放减少。
本研究结果表明，老年人短期LSP通过在细胞水平调节氧化应激发挥抗衰老作用。这些发现的意义可能与作为抗衰老方法的预后和治疗策略有关。
