Wang Yuye, Zhang Heyu, Hao Yuehan, Jin Feng, Tang Ling, Xu Xiaoxue, He Zhiyi, Wang Yanzhe
Department of Neurology, Key Laboratory for Neurological Big Data of Liaoning Province, The First Affiliated Hospital of China Medical University, Shenyang, Liaoning, 110001, China.
Department of Neurology, China-Japan Friendship Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100029, China.
Heliyon. 2024 Aug 8;10(16):e35864. doi: 10.1016/j.heliyon.2024.e35864. eCollection 2024 Aug 30.
Circular RNAs (circRNAs) are involved in several neurological disorders; however, the mechanisms underlying their involvement remain to be clarified. We attempted to explore the expression profiles of circRNAs and their potential functions and mechanisms in the pathogenesis of intracerebral hemorrhage (ICH) in Northern Chinese males. The microarray results showed that 50 circRNAs were significantly upregulated, while 194 circRNAs were significantly downregulated in ICH patients compared with healthy controls (p < 0.05). After bioinformatics analysis, a circRNA-microRNA-messenger RNA network and a protein-protein interaction network were constructed. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses showed that the neurotrophin signaling pathway, long-term potentiation, and the mitogen-activated protein kinase pathway are potentially implicated in ICH pathophysiology. The quantitative real-time polymerase chain reaction results revealed that hsa-circ-0090829 was significantly downregulated in ICH. The receiver operating characteristic curve analysis showed that the area under the curve of hsa-circ-0090829 between ICH and healthy controls was 0.807. Furthermore, the dual-luciferase assay showed that hsa-circ-0090829 sponged miR-526b-5p. This study reports the altered expression of circRNAs and identifies the potential functions of these circRNAs in ICH. Our results may facilitate further mechanistic research on circRNAs in ICH and provide probable novel diagnostic biomarkers and therapeutic targets for ICH.
环状RNA（circRNAs）与多种神经系统疾病有关；然而，其涉及的潜在机制仍有待阐明。我们试图探索环状RNA在中国北方男性脑出血（ICH）发病机制中的表达谱及其潜在功能和机制。微阵列结果显示，与健康对照相比，ICH患者中有50种环状RNA显著上调，194种环状RNA显著下调（p<0.05）。经过生物信息学分析，构建了一个环状RNA-微小RNA-信使RNA网络和一个蛋白质-蛋白质相互作用网络。基因本体论和京都基因与基因组百科全书富集分析表明，神经营养因子信号通路、长时程增强和丝裂原活化蛋白激酶通路可能与ICH的病理生理学有关。定量实时聚合酶链反应结果显示，hsa-circ-0090829在ICH中显著下调。受试者工作特征曲线分析表明，hsa-circ-0090829在ICH和健康对照之间的曲线下面积为0.807。此外，双荧光素酶测定表明，hsa-circ-0090829可吸附miR-526b-5p。本研究报告了环状RNA的表达变化，并确定了这些环状RNA在ICH中的潜在功能。我们的结果可能有助于进一步开展关于ICH中环状RNA的机制研究，并为ICH提供可能的新型诊断生物标志物和治疗靶点。
