Inoue Takahiro, Takamatsu Yasuyuki, Okamura Misato, Maejima Hiroshi
Graduate School of Health Sciences, Hokkaido University, Kita 12 Nishi 5, Kita-ku, Sapporo 060-0812, Japan; Research Fellow of Japan Society for the Promotion of Science, 5-3-1 Kojimachi, Chiyoda-ku, Tokyo 102-0083, Japan.
Department of Rehabilitation Science, Faculty of Health Sciences, Hokkaido University, Kita 12 Nishi 5, Kita-ku, Sapporo 060-0812, Japan.
Brain Res. 2021 Sep 15;1767:147536. doi: 10.1016/j.brainres.2021.147536. Epub 2021 May 28.
Intracerebral hemorrhage (ICH) is a subtype of stroke that causes major motor impairments. Brain-derived neurotrophic factor (BDNF) is known to have important roles in neuroplasticity and beneficially contributes to stroke recovery. This study aimed to characterize BDNF expression in the motor cortex after ICH and investigate the relationship between cortical BDNF expression and behavioral outcomes using an ICH rat model. Wistar rats were divided into two groups: a SHAM group (n = 7) and an ICH group (n = 8). ICH was induced by the injection of collagenase into the left striatum near the internal capsule. For behavioral assessments, the cylinder test and open field test were performed before surgery and 3 days, 1 week, 2 weeks, and 4 weeks after surgery. Following the behavioral assessments at 4 weeks, BDNF expression in the ipsilateral and contralateral motor cortex was assayed using RT-PCR and ELISA methods. There was no significant difference in either cortical BDNF mRNA or protein expression levels between the SHAM and ICH groups. However, the asymmetry index of BDNF mRNA expression between the ipsilateral and contralateral hemispheres shifted to the ipsilateral hemisphere after ICH. Furthermore, the ipsilateral cortical BDNF mRNA expression level positively correlated with motor function in the affected forelimb after ICH. This study describes for the first time that cortical BDNF mRNA expression is related to post-ICH motor impairment. These results highlight the importance of assessing the interhemispheric laterality of BDNF expression and could help develop novel treatment strategies for BDNF-dependent recovery after ICH.
脑出血（ICH）是中风的一种亚型，可导致严重的运动障碍。脑源性神经营养因子（BDNF）在神经可塑性中发挥重要作用，并对中风恢复有有益作用。本研究旨在描述脑出血后运动皮层中BDNF的表达特征，并使用脑出血大鼠模型研究皮层BDNF表达与行为结果之间的关系。将Wistar大鼠分为两组：假手术组（n = 7）和脑出血组（n = 8）。通过向靠近内囊的左侧纹状体内注射胶原酶诱导脑出血。在手术前以及手术后3天、1周、2周和4周进行行为评估，包括圆筒试验和旷场试验。在4周进行行为评估后，使用RT-PCR和ELISA方法检测同侧和对侧运动皮层中BDNF的表达。假手术组和脑出血组之间的皮层BDNF mRNA或蛋白表达水平均无显著差异。然而，脑出血后同侧和对侧半球之间BDNF mRNA表达的不对称指数向同侧半球偏移。此外，脑出血后同侧皮层BDNF mRNA表达水平与患侧前肢的运动功能呈正相关。本研究首次描述了皮层BDNF mRNA表达与脑出血后运动障碍有关。这些结果突出了评估BDNF表达半球间不对称性的重要性，并有助于开发脑出血后依赖BDNF恢复的新治疗策略。
