Lukheli Rangani Tracy, Tavengwa Nikita Tawanda, Mokgehle Tebogo Mphatlalala
Department of Chemistry Faculty of Science Engineering and Agriculture University of Venda Thohoyandou South Africa.
Anal Sci Adv. 2024 Aug 24;5(7-8):e2400006. doi: 10.1002/ansa.202400006. eCollection 2024 Aug.
Enterolactone, coumaric acid and vitexin are polyphenolic compounds present in a variety of fruits, vegetables, cereals and plants. These bioactive compounds are in high demand due to their antioxidant property in various tissues and organs. The purpose of this study was to develop a simultaneous extraction method, an aqueous two-phase extraction (ATPE) method, that would enable the extraction of these compounds from . This environmentally friendly extraction method only applied water and ethanol as extraction solvents for these analytes from the plant matrix. After phase separation, the analytes were salted-out from the aqueous phase into the organic phase with the aid of a chaotrope (NaCl) or kosmotrope (NaCO). Thereafter, the analytes were withdrawn by a micro-pipette for analysis on the high-performance liquid chromatography-photodiode array detector. Optimization was conducted using a central composite design, where three parameters were examined which involved percentage ethanol, centrifugation time and salt type. Generally, the optimized conditions for extraction were an ethanol percentage of 100% and a centrifugation time of 10 min, which yielded concentrations of 2942, 23,823 and 8881 mg kg for enterolactone, vitexin and coumaric acid, respectively, in the presence of a kosmotrope. The optimized conditions of extraction in the presence of chaotrope were an ethanol percentage of 66% and a centrifugation time of 10 min with concentrations of 6727, 20,833 and 8618 mg kg for enterolactone, vitexin and coumaric acid, respectively. The ATPE method involving NaCO was a better extractant of all the compounds studied relative to that of NaCl. The superior extraction capability of NaCO in ATPE could serve as a prototype for the development of efficient extraction methods to meet the high demand for medicinal compounds derived from natural products.
肠内酯、香豆酸和牡荆素是存在于多种水果、蔬菜、谷物和植物中的多酚类化合物。由于这些生物活性化合物在各种组织和器官中具有抗氧化特性，因此对它们的需求量很大。本研究的目的是开发一种同时萃取方法，即双水相萃取（ATPE）方法，该方法能够从……中萃取这些化合物。这种环境友好型萃取方法仅使用水和乙醇作为从植物基质中萃取这些分析物的溶剂。相分离后，借助离液剂（氯化钠）或促溶剂（碳酸钠）将分析物从水相盐析到有机相中。此后，用微量移液器取出分析物，用于在高效液相色谱 - 光电二极管阵列检测器上进行分析。使用中心复合设计进行优化，其中考察了三个参数，包括乙醇百分比、离心时间和盐的类型。一般来说，优化的萃取条件是乙醇百分比为100%，离心时间为10分钟，在存在促溶剂的情况下，肠内酯、牡荆素和香豆酸的浓度分别为2942、23823和8881毫克/千克。在存在离液剂的情况下，优化的萃取条件是乙醇百分比为66%，离心时间为10分钟，肠内酯、牡荆素和香豆酸的浓度分别为6727、20833和8618毫克/千克。相对于氯化钠，涉及碳酸钠的双水相萃取方法是所研究的所有化合物的更好萃取剂。碳酸钠在双水相萃取中卓越的萃取能力可作为开发高效萃取方法的原型，以满足对源自天然产物的药用化合物的高需求。
