评价一种混沌溶剂和规则溶剂在从. 叶子中提取 Solasodine 的 PHW-ATPE 的多变量优化中的作用。

Evaluation of a Chaotrope and Kosmotrope in the Multivariate Optimization of PHW-ATPE of Solasodine from Leaves of .

机构信息

Department of Chemistry, Faculty of Science, Engineering and Agriculture, University of Venda, Private Bag X5050, Thohoyandou 0950, South Africa.

Department of Biochemistry, Faculty of Science, Engineering and Agriculture, University of Venda, Private Bag X5050, Thohoyandou 0950, South Africa.

出版信息

Molecules. 2022 Aug 29;27(17):5547. doi: 10.3390/molecules27175547.

DOI:10.3390/molecules27175547

PMID:36080313

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9457875/

Abstract

A hyphenated pressurized hot water—aqueous two-phase extraction (PHW-ATPE) method was applied to extract solasodine from Solanum mauritianum (S. mauritianum). A central composite design (CCD) was applied to determine the optimal conditions for the extraction of solasodine. The parameters evaluated included the percentage concentration of salt (NaCl or Na2CO3) and temperature. The fit of the central composite design response surface model for PHW-ATPE to the data generated a model with a good quadratic fit (R2 = 0.901). The statistically significant (p < 0.05) parameters, such as the linear and quadratic effects of the concentration of salt (%) powder, had a significant impact on the extraction of solasodine. The application of multiply charged salts such as Na2CO3 (kosmotrope) was shown to be a comparably better extractant of solasodine than NaCl (chaotrope) due to the salting-out effect. The optimized conditions for extraction of solasodine with NaCl or Na2CO3 were a temperature of 80 °C at a salt concentration of 20%. The maximum extraction of solasodine was 300.79 mg kg−1 and 162.34 mg kg−1 for Na2CO3 and NaCl, respectively.

摘要

一种双相加压热水-水相萃取（PHW-ATPE）方法被应用于从茄科植物（Solanum mauritianum）中提取澳洲茄碱。采用中心复合设计（CCD）确定了萃取澳洲茄碱的最佳条件。评估的参数包括盐（NaCl 或 Na2CO3）的百分比浓度和温度。PHW-ATPE 的中心复合设计响应面模型对所生成数据的拟合具有良好的二次拟合（R2 = 0.901）。盐（%粉末）浓度的线性和二次效应等统计学上显著（p < 0.05）的参数对澳洲茄碱的萃取有显著影响。多电荷盐如 Na2CO3（助溶盐）的应用由于盐析效应，被证明是比 NaCl（抗溶盐）更好的澳洲茄碱萃取剂。使用 NaCl 或 Na2CO3 萃取澳洲茄碱的最佳条件为盐浓度为 20%时，温度为 80°C。使用 Na2CO3 和 NaCl 的澳洲茄碱最大萃取量分别为 300.79 mg kg−1 和 162.34 mg kg−1。