Department of General and Inorganic Chemistry, Faculty of Chemistry, Aristotle University of Thessaloniki, GR-54124 Thessaloniki, Greece.
Dalton Trans. 2024 Sep 18;53(36):15215-15235. doi: 10.1039/d4dt01846j.
Fourteen cobalt(II) complexes with the non-steroidal anti-inflammatory drugs sodium meclofenamate, tolfenamic acid, mefenamic acid, naproxen, sodium diclofenac, and diflunisal were prepared in the presence or absence of a series of nitrogen-donors (namely imidazole, pyridine, 3-aminopyridine, neocuproine, 2,2'-bipyridine, 1,10-phenanthroline and 2,2'-bipyridylamine) as co-ligands and were characterised by spectroscopic and physicochemical techniques. Single-crystal X-ray crystallography was employed to determine the crystal structure of eight complexes. The biological profile of the complexes was investigated regarding their interaction with serum albumins and DNA, and their antioxidant potency. The interaction of the compounds with calf-thymus DNA takes place intercalation. The ability of the complexes to cleave pBR322 plasmid DNA at the concentration of 500 μM is rather low. The complexes demonstrated tight and reversible binding to human and bovine serum albumins and the binding site of bovine serum albumin was also examined. In order to assess the antioxidant activity of the compounds, the scavenging activity towards free radicals, namely 1,1-diphenyl-picrylhydrazyl and 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid), and their ability to reduce HO were studied.
十四种钴（II）配合物，含有非甾体类抗炎药（如甲氯芬那酸、托芬那酸、甲芬那酸、萘普生、双氯芬酸钠和二氟尼柳），在存在或不存在一系列氮供体（如咪唑、吡啶、3-氨基吡啶、新铜试剂、2,2'-联吡啶、1,10-菲咯啉和 2,2'-联吡啶胺）作为共配体的情况下被制备，并通过光谱和物理化学技术进行了表征。采用单晶 X 射线晶体学确定了其中八个配合物的晶体结构。研究了这些配合物与血清白蛋白和 DNA 的相互作用以及它们的抗氧化能力，以评估它们的生物特性。这些化合物与小牛胸腺 DNA 的相互作用方式是嵌入。这些配合物在 500μM 浓度下切割 pBR322 质粒 DNA 的能力相当低。这些配合物与人血清白蛋白和牛血清白蛋白具有紧密和可逆的结合能力，并且还检查了牛血清白蛋白的结合位点。为了评估化合物的抗氧化活性，研究了它们对自由基（如 1,1-二苯基-2-苦基肼基和 2,2'-联氨-双（3-乙基苯并噻唑啉-6-磺酸））的清除活性以及它们还原 HO 的能力。
