Lemos Mateus L P, do Monte Daniel F M, Leite Elma L, Egito Antônio S, Olbrich Karina M, Oliveira Celso J B
Department of Animal Science, College for Agricultural Sciences, Federal University of Paraiba (CCA/UFPB), Areia, PB, 58397-000, Brazil.
Embrapa Goats and Sheep, Northeast Regional Center, R. Osvaldo Cruz 1143, Campina Grande, PB, 58428-09, Brazil.
Braz J Microbiol. 2024 Dec;55(4):3141-3146. doi: 10.1007/s42770-024-01480-1. Epub 2024 Aug 26.
Probiogenomics can provide important insights bout probiotic candidate bacteria. This study aimed to perform an in-depth genomic characterization of the probiotic candidate Lactiplantibacillus plantarum CNPC024 to investigate its probiosis mechanisms, identify metabolic pathways that might benefit the host, and improve the safety assessment for this strain to be effectively used as a probiotic. After whole-genome sequencing in Illumina MiSeq platform, the de novo genome assembly resulted in a 3.2 Mb draft genome. According to the Average Nucleotide Identity (ANI) analysis with 46 randomly validated probiotic LAB belonging to the Lactobacillaceae family, the strain showed a 99% nucleotide identity with other L. plantarum probiotic species. We identified a set of determinants conferring tolerance to bile salts and low pH conditions, as well as temperature, oxidative and osmotic stressors via the glutathione-glutaredoxin system (Grxs). As a β‑galactosidase‑producing strain, it has the potential to be used in fermented dairy products for lactose-intolerant individuals. There were no significant hits for transferable antibiotic-resistance genes. We also identified gene clusters associated with production of bacteriocins (plantaricins E, F and K). Lastly, we detected metabolic pathways associated with the production of tryptophan-derived metabolites that could potentially modulate the host's immune responses.
益生菌基因组学可以为益生菌候选菌株提供重要见解。本研究旨在对益生菌候选植物乳杆菌CNPC024进行深入的基因组特征分析，以研究其益生机制，确定可能有益于宿主的代谢途径，并改进该菌株作为益生菌有效使用的安全性评估。在Illumina MiSeq平台上进行全基因组测序后，从头基因组组装得到了一个3.2 Mb的基因组草图。通过与46株随机验证的属于乳杆菌科的益生菌LAB进行平均核苷酸同一性（ANI）分析，该菌株与其他植物乳杆菌益生菌种显示出99%的核苷酸同一性。我们通过谷胱甘肽-谷氧还蛋白系统（Grxs）鉴定出了一组赋予对胆盐、低pH条件以及温度、氧化和渗透应激耐受性的决定因素。作为一株产生β-半乳糖苷酶的菌株，它有潜力用于生产适合乳糖不耐受个体的发酵乳制品。未发现可转移抗生素抗性基因的显著匹配。我们还鉴定出了与细菌素（植物乳杆菌素E、F和K）产生相关的基因簇。最后，我们检测到了与色氨酸衍生代谢物产生相关的代谢途径，这些代谢物可能会调节宿主的免疫反应。
