探索植物乳杆菌NMGL2的多方面益生菌潜力，研究其抗菌抗性谱和细菌素产生情况。
Exploring the multifaceted probiotic potential of Lactiplantibacillus plantarum NMGL2, investigating its antimicrobial resistance profiles and bacteriocin production.
作者信息
Aziz Tariq, Shabbir Muhammad Aqib, Sarwar Abid, Khan Ayaz Ali, Zhao Liqing, Yang Zhennai, Shami Ashwag, Alwethaynani Maher S, Al-Asmari Fahad, Alghamdi Abeer M, Al-Joufi Fakhria A
机构信息
Department of Food Science and Technology College of Chemistry and Environmental Engineering Shenzhen University, Shenzhen, Guangdong, China; School of Biomedical Engineering Shenzhen University, Shenzhen, Guangdong, China; Key Laboratory of Geriatric Nutrition and Health of Ministry of Education, Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Engineering and Technology Research Center of Food Additives, Beijing Technology and Business University, No. 11 Fucheng Road, Hai-Dian District, Beijing 100048, China.
Department of Biotechnology, Faculty of Biological Sciences, Lahore University of Biological & Applied Sciences, 53400 Lahore, Pakistan.
出版信息
J Microbiol Methods. 2025 Jun 20;236:107178. doi: 10.1016/j.mimet.2025.107178.
BACKGROUND
Lactiplantibacillus plantarum is widely recognized for its probiotic and antimicrobial properties, making it a valuable candidate for food and clinical applications. Genomic characterization provides deeper insight into its potential health benefits and safety profile.
AIM
This study aimed to sequence and analyze the genome of L. plantarum NMGL2 to evaluate its antimicrobial resistance, probiotic potential, and genetic suitability for biotechnological applications.
METHODS
The genomic DNA of L. plantarum NMGL2 was extracted and sequenced using Illumina technology. Genome assembly and annotation were performed, followed by gene prediction using Prokka and identification of antimicrobial resistance genes, virulence factors, and probiotic markers via BLAST. Metagenomic analysis of gut microbiota samples and phylogenetic analysis were conducted to assess strain relationships with other L. plantarum isolates.
RESULTS
The genome analysis revealed approximately 3000 protein-coding genes, including those encoding bile salt hydrolase, antimicrobial peptides, and antibiotic resistance determinants. Phylogenetic analysis showed that NMGL2 is closely related to other probiotic L. plantarum strains, supporting its probiotic characteristics and its potential role in combating pathogens.
CONCLUSION
L. plantarum NMGL2 demonstrates promising probiotic traits and carries genes that support its application in food safety and clinical contexts. Further, in vivo studies are needed to validate its health benefits and ensure safety, particularly in treating gastrointestinal disorders.
背景
植物乳杆菌因其益生菌和抗菌特性而被广泛认可，使其成为食品和临床应用中有价值的候选菌株。基因组特征分析能更深入地了解其潜在的健康益处和安全性。
目的
本研究旨在对植物乳杆菌NMGL2的基因组进行测序和分析，以评估其抗菌抗性、益生菌潜力以及在生物技术应用中的遗传适应性。
方法
提取植物乳杆菌NMGL2的基因组DNA，并使用Illumina技术进行测序。进行基因组组装和注释，随后使用Prokka进行基因预测，并通过BLAST鉴定抗菌抗性基因、毒力因子和益生菌标记。对肠道微生物群样本进行宏基因组分析并进行系统发育分析，以评估该菌株与其他植物乳杆菌分离株的关系。
结果
基因组分析揭示了大约3000个蛋白质编码基因，包括那些编码胆汁盐水解酶、抗菌肽和抗生素抗性决定因素的基因。系统发育分析表明，NMGL2与其他益生菌植物乳杆菌菌株密切相关，支持其益生菌特性及其在对抗病原体中的潜在作用。
结论
植物乳杆菌NMGL2表现出有前景的益生菌特性，并携带支持其在食品安全和临床环境中应用的基因。此外，需要进行体内研究以验证其健康益处并确保安全性，特别是在治疗胃肠道疾病方面。
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